• Product nameAnti-Rel B antibody [EP614Y]
    See all Rel B primary antibodies
  • Description
    Rabbit monoclonal [EP614Y] to Rel B
  • Tested applicationsSuitable for: ICC/IF, WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C term of RelB (Human)

  • Positive control
    • WB: Raji and Daudi cell lysate IP: Daudi cell lysate ICC/IF: Raji cells FC: Raji cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab33907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/2000 - 1/20000. Predicted molecular weight: 62 kDa.
Flow Cyt 1/70.

For unpurified, use 1/1000.

ab172730 - Rabbit monoclonal IgG is suitable for use as an isotype control with this antibody.

IP 1/40 - 1/50.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionNF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
    • Sequence similaritiesContains 1 RHD (Rel-like) domain.
    • DomainBoth N- and C-terminal domains are required for transcriptional activation.
    • Post-translational
      Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
    • Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • I REL antibody
      • I-Rel antibody
      • IREL antibody
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
      • relB antibody
      • RELB_HUMAN antibody
      • Reticuloendotheliosis viral oncogene homolog B antibody
      • Transcription factor Rel B antibody
      • Transcription factor RelB antibody
      • v rel avian reticuloendotheliosis viral oncogene homolog B antibody
      • v rel reticuloendotheliosis viral oncogene homolog B antibody
      see all

    Anti-Rel B antibody [EP614Y] images

    • All lanes : Anti-Rel B antibody [EP614Y] (ab33907) at 1/2000 dilution (purified)

      Lane 1 : Raji cell lysate
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 20 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 62 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • ab33907 (purified) at 1/20 immunoprecipitating Rel B in 10 μg Daudi cell lysate (Lanes 1 and 2, observed at 70 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/1000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
    • Overlay histogram showing Raji cells fixed in 80% methanol and stained with purified ab33907 at a dilution of 1/70 (red line). The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit at a dilution of 1/500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).

    • Immunofluorescence staining of Raji cells with purified ab33907 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4 % PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab33907 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Overlay histogram showing Raji cells stained with unpurified ab33907 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab33907, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-Rel B antibody [EP614Y] (ab33907) at 1/20000 dilution (unpurified) + Raji Cell Lysate

      Predicted band size : 62 kDa
      Observed band size : 62 kDa

    References for Anti-Rel B antibody [EP614Y] (ab33907)

    This product has been referenced in:
    • Chen X  et al. The NF-kappaB factor RelB and histone H3 lysine methyltransferase G9a directly interact to generate epigenetic silencing in endotoxin tolerance. J Biol Chem 284:27857-65 (2009). IP ; Human . Read more (PubMed: 19690169) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab33907.
    Please use the links above to contact us or submit feedback about this product.