Anti-Rel B (phospho S573) antibody (ab47366)

Overview

  • Product name
    Anti-Rel B (phospho S573) antibody
    See all Rel B primary antibodies
  • Description
    Rabbit polyclonal to Rel B (phospho S573)
  • Specificity
    ab47366 detects endogenous levels of Rel B only when phosphorylated at serine 573.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Rel B (phospho S573). Synthesized peptide derived from human RelB around the phosphorylation site of Ser573.
    Database link: Q01201

  • Positive control
    • LoVo and RAW 264.7 cells, breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab47366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
  • Sequence similarities
    Contains 1 RHD (Rel-like) domain.
  • Domain
    Both N- and C-terminal domains are required for transcriptional activation.
  • Post-translational
    modifications
    Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > centrosome. Co-localizes with NEK6 in the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • I REL antibody
    • I-Rel antibody
    • IREL antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
    • relB antibody
    • RELB_HUMAN antibody
    • Reticuloendotheliosis viral oncogene homolog B antibody
    • Transcription factor Rel B antibody
    • Transcription factor RelB antibody
    • v rel avian reticuloendotheliosis viral oncogene homolog B antibody
    • v rel reticuloendotheliosis viral oncogene homolog B antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47366. Image on the left is tissue untreated with immunising peptide; image on the right is tissue treated with immunising peptide.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47366. Image on the left is tissue untreated with immunising peptide; image on the right is tissue treated with immunising peptide.
  • All lanes : Anti-Rel B (phospho S573) antibody (ab47366) at 1/500 dilution

    Lane 1 : LoVo (Human colorectal adenocarcinoma cell line) cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) cell lysate with phospho peptide


    Predicted band size : 62 kDa

References

ab47366 has not yet been referenced specifically in any publications.

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