- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Relaxin 3 receptor 1 antibody
See all Relaxin 3 receptor 1 primary antibodies -
DescriptionRabbit polyclonal to Relaxin 3 receptor 1
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human GPCR SALPR.
(Peptide available as ab89370.)
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Positive control
- This antibody gave a positive signal in the SKNSH whole cell lysate and the following tissue lysates: Human brain; Human brain membrane; Human spinal cord; Human kidney.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab72914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
Target
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FunctionReceptor for RNL3/relaxin-3. Binding of the ligand inhibit cAMP accumulation.
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Tissue specificityExpressed predominantly in brain regions. Highest expression in substantia nigra and pituitary, followed by hippocampus, spinal cord, amygdala, caudate nucleus and corpus callosum, quite low level in cerebellum. In peripheral tissues, relatively high levels in adrenal glands, low levels in pancreas, salivary gland, placenta, mammary gland and testis.
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Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
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Cellular localizationCell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 51289 Human
- Entrez Gene: 239336 Mouse
- Entrez Gene: 294807 Rat
- Omim: 609445 Human
- SwissProt: Q9NSD7 Human
- SwissProt: Q8BGE9 Mouse
- Unigene: 170146 Human
- Unigene: 209312 Mouse
see all -
Alternative names
- G protein coupled receptor GPCR135 antibody
- G protein coupled receptor SALPR antibody
- G protein-coupled receptor SALPR antibody
see all
Images
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Western blot - GPCR SALPR antibody (ab72914)All lanes : Anti-Relaxin 3 receptor 1 antibody (ab72914) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 3 : Human spinal cord tissue lysate - total protein (ab29188)
Lane 4 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lane 5 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 50 kDa (why is the actual band size different from the predicted?)
Additional bands at: 15 kDa, 37 kDa, 49 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Relaxin-3 receptor 1 (RXFP3)has multiple glycosylation sites (SwissProt) as can be seen by the characteristic banding observed at 49 kDa.
Protocols
References
ab72914 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"