Anti-RELL1 antibody (ab121394)
Key features and details
- Rabbit polyclonal to RELL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RELL1 antibody -
Description
Rabbit polyclonal to RELL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 127-257 of Human RELL1 (UniProt ID: Q8IUW5).
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Positive control
- WB: RH-30 cell line. IHC-P: Human skin, gastrointestinal, lymphoid and cerebral cortex tissue. ICC/IF: Human cell line U-2 OS.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Tissue specificity
Widely expressed. Expressed at highest levels in the placenta, skeletal muscle, spleen and testis. -
Sequence similarities
Belongs to the RELT family. -
Post-translational
modificationsPhosphorylated in vitro by OXSR1. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 768211 Human
- Omim: 611212 Human
- SwissProt: Q8IUW5 Human
- Unigene: 283378 Human
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Alternative names
- RELL1 antibody
- RELL1_HUMAN antibody
- RELT-like 1 antibody
- RELT-like protein 1 antibody
Images
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ab121394, at 4 µg/ml, staining RELL1 in Human cell line U-2 OS by Immunofluorescence.
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Anti-RELL1 antibody (ab121394) at 0.4 µg/ml + human cell line RH-30
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Immunohistochemical analysis of human gastrointestinal tissue labeling RELL1 with ab121394 at a 1/200 dilution.
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Immunohistochemical analysis of human skin tissue labeling RELL1 with ab121394 at a 1/200 dilution.
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Immunohistochemical analysis of human lymphoid tissue labeling RELL1 with ab121394 at a 1/200 dilution.
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Immunohistochemical analysis of human cerebral cortex tissue labeling RELL1 with ab121394 at a 1/200 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121394 has not yet been referenced specifically in any publications.