Overview

  • Product name
    Anti-RELL1 antibody
  • Description
    Rabbit polyclonal to RELL1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen, corresponding to amino acids 127-257 of Human RELL1 (UniProt ID: Q8IUW5).

  • Positive control
    • WB: U-251 MG IHC-P: Human skin tissue ICC/IF: Human cell line U-2 OS

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

Images

  • All lanes : Anti-RELL1 antibody (ab121394) at 1/250 dilution

    Lane 1 : RT-4
    Lane 2 : U-251 MG
    Lane 3 : Human plasma
    Lane 4 : Human liver
    Lane 5 : Human tonsil

    Developed using the ECL technique
  • ab121394, at a 1/35 dilution, staining RELL1 in paraffin embedded Human skin tissue by Immunohistochemistry.
  • ab121394, at 4 µg/ml, staining RELL1 in Human cell line U-2 OS by Immunofluorescence.

References

ab121394 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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