Overview

  • Product nameAnti-RELM alpha antibody
    See all RELM alpha primary antibodies
  • Description
    Rabbit polyclonal to RELM alpha
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein (Mouse)

Properties

  • FormLyophilised:Reconstitute in sterile water to 0.2 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab39626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
ELISA Use a concentration of 0.5 µg/ml.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.

Target

  • FunctionProbable hormone.
  • Tissue specificityHighest levels in adipose tissue.
  • Sequence similaritiesBelongs to the resistin/FIZZ family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cysteine-rich secreted protein A12-gamma antibody
    • Cysteine-rich secreted protein FIZZ1 antibody
    • FIZZ1 antibody
    • Parasite-induced macrophage novel gene 1 protein antibody
    • RELMalpha antibody
    • Resistin like alpha antibody
    • Resistin-like alpha antibody
    • RETNA_MOUSE antibody
    • Retnla antibody
    see all

Anti-RELM alpha antibody images

  • ab39626 at 4 ng/ml staining murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse) by IHC-P. The primary antibody was incubated overnight at 4˚C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit IgG.  Heat induced antigen retrieval with a 100mM Citric Acid was used.
  • ab39626 staining RELM alpha in murine liver tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde and then blocked using 5% BSA for 30 minutes. Samples were then incubated with ab39626 at a 1/20 dilution for 1 hour. The secondary used was a biotinylated goat anti-rabbit IgG used at a 1/200 dilution.

    See Abreview

References for Anti-RELM alpha antibody (ab39626)

This product has been referenced in:
  • Klug F  et al. Low-dose irradiation programs macrophage differentiation to an iNOS?/M1 phenotype that orchestrates effective T cell immunotherapy. Cancer Cell 24:589-602 (2013). WB ; Mouse . Read more (PubMed: 24209604) »
  • Sun L  et al. New concepts of IL-10-induced lung fibrosis: fibrocyte recruitment and M2 activation in a CCL2/CCR2 axis. Am J Physiol Lung Cell Mol Physiol 300:L341-53 (2011). Read more (PubMed: 21131395) »

See all 6 Publications for this product

Product Wall

We have not suggested a positive control for ab39626. However, RELM alpha is predominantly found in adipose tissue therefore I could suggest Adipose (Mouse) Whole Cell Lysate - normal tissue (ab29417) as a positive control.

Thank you very much for contacting us with your enquiry.

I apologize for the delay getting this SDS for you. There was an IT issue with the PDF on the website, but this has now been corrected. I have also attached the PDF version for you. Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheets and for the inconvenience t...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Perineal adipose tissue)
Specification Perineal adipose tissue
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2012

Thank you for contacting us and your interest in our products.


As discussed over the phone ab8918isamouse monoclonaland canbe used with an anti-mouse IgG secondary antibody.


ab39626 and ab9969 are bothrabbit polyclonals ...

Read More

Thank you for contacting us. The complete datasheet for each product can be found online. For ab39626 it is: http://www.abcam.com/RELM-alpha-antibody-ab39626.html There is also a button to create a pdf-file. I have attached this file to this email. ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jun 22 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (bone marrow derived macrophages)
Loading amount 40 µg
Specification bone marrow derived macrophages
Treatment DMSO and IL-4 10 ng/ml
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"