Our Abpromise guarantee covers the use of ab39626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 0.5 µg/ml.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.|
ab39626 staining RELM alpha in Mouse cecum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citric acid. Samples were incubated with primary antibody (4 ng/ml) overnight at 4°C. A Cy3-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.