Overview

  • Product nameAnti-REM2 antibody
  • Description
    Mouse polyclonal to REM2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human REM2 protein (AAH35663, 1 a.a. ~ 331 a.a).

  • Positive control
    • HeLa cells. REM2 transfected 293T cell lsyate.

Properties

Applications

Our Abpromise guarantee covers the use of ab73088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notesICC/IF: Use at a concentration of 10 µg/ml. Protein A purify ab73088 before starting ICC/IF procedure.
    WB: 1/500 - 1/1000. Predicted molecular weight: 36 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceREM2, a Ras-like GTPase, may be involved in increasing endothelial cell proliferation and the induction of angiogenesis. REM2 belongs to the small GTPase superfamily, RGK family. It binds GTP saturably and exhibits a low intrinsic rate of GTP hydrolysis.
    • Cellular localizationCell Membrane
    • Database links
    • Alternative names
      • FLJ38964 antibody
      • GTP binding protein REM 2 antibody
      • Rad and Gem like GTP binding protein 2 antibody
      • Rad and Gem related 2 antibody
      • Rad and gem related GTP binding protein 2 antibody
      • RAS (RAD and GEM) like GTP binding 2 antibody
      • REM 2 antibody
      see all

    Anti-REM2 antibody images

    • Immunofluorescent staining of REM2 in HeLa cells using Protein A purified ab73088 at 10µg/ml.
    • All lanes : Anti-REM2 antibody (ab73088) at 1/500 dilution

      Lane 1 : REM2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 36 kDa
      Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    References for Anti-REM2 antibody (ab73088)

    ab73088 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (HEK and HeLa cells transfected with Rem2 plasmid)
    Loading amount 10 µg
    Specification HEK and HeLa cells transfected with Rem2 plasmid
    Gel Running Conditions Reduced Denaturing (10% resolving, 4.5% stacking gel)
    Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Van Lu

    Verified customer

    Submitted Feb 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"