Overview

  • Product name
    Anti-RENBP antibody
  • Description
    Rabbit polyclonal to RENBP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Pig, Baboon
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human RENBP.

    (Peptide available as ab88230.)

  • Positive control
    • This antibody gave a positive signal in human liver tissue lysate and the following whole cell lysates: HeLa; HepG2; Jurkat; HEK293; Ramos; SHSY5Y.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Catalyzes the interconversion of N-acetylglucosamine to N-acetylmannosamine. Binds to renin forming a protein complex called high molecular weight (HMW) renin and inhibits renin activity.
  • Sequence similarities
    Belongs to the N-acylglucosamine 2-epimerase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGE antibody
    • GlcNAc 2 epimerase antibody
    • GlcNAc 2-epimerase antibody
    • N acetyl D glucosamine 2 epimerase antibody
    • N-acetyl-D-glucosamine 2-epimerase antibody
    • N-acylglucosamine 2-epimerase antibody
    • RBP antibody
    • RENBP antibody
    • RENBP_HUMAN antibody
    • Renin binding protein antibody
    • Renin-binding protein antibody
    • RnBP antibody
    see all

Images

  • All lanes : Anti-RENBP antibody (ab77081) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References

ab77081 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab77081.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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