Recombinant Anti-Renin antibody [EPR6433] (ab125012)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6433] to Renin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Renin antibody [EPR6433]
See all Renin primary antibodies -
Description
Rabbit monoclonal [EPR6433] to Renin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Renin aa 50-150. The exact sequence is proprietary.
Database link: P00797 -
Positive control
- K562, 293T, and HepG2 whole cell lysate (ab7900). K562 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 1.70 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6433 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
|
Notes |
---|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody |
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa). |
Target
-
Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
Involvement in disease
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
-
Database links
- Entrez Gene: 5972 Human
- Omim: 179820 Human
- SwissProt: P00797 Human
- Unigene: 3210 Human
-
Alternative names
- Angiotensin forming enzyme antibody
- Angiotensin forming enzyme precursor antibody
- Angiotensinogenase antibody
see all
Images
-
All lanes : Anti-Renin antibody [EPR6433] (ab125012) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of permeabilized K562 cells using ab125012 at 1/10 shown in red or a rabbit IgG negative control shown in green.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab125012 has not yet been referenced specifically in any publications.