Recombinant
RabMAb

Anti-Renin antibody [EPR6433] (ab125012)

Overview

  • Product name
    Anti-Renin antibody [EPR6433]
    See all Renin primary antibodies
  • Description
    Rabbit monoclonal [EPR6433] to Renin
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Renin aa 50-150.
    Database link: P00797

  • Positive control
    • K562, 293T, and HepG2 cell lysates. K562 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab125012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
    • Involvement in disease
      Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
      Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.
    • Sequence similarities
      Belongs to the peptidase A1 family.
    • Cellular localization
      Secreted. Membrane. Associated to membranes via binding to ATP6AP2.
    • Information by UniProt
    • Database links
    • Alternative names
      • Angiotensin forming enzyme antibody
      • Angiotensin forming enzyme precursor antibody
      • Angiotensinogenase antibody
      • Angiotensinogenase precursor antibody
      • FLJ10761 antibody
      • HNFJ2 antibody
      • REN antibody
      • Ren1 antibody
      • RENI_HUMAN antibody
      • Renin antibody
      • Renin precursor renal antibody
      see all

    Images

    • All lanes : Anti-Renin antibody [EPR6433] (ab125012) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : 293T lysate
      Lane 3 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized K562 cells using ab125012 at 1/10 shown in red or a rabbit IgG negative control shown in green.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab125012 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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