Overview

  • Product nameAnti-RENT1/hUPF1 antibody
    See all RENT1/hUPF1 primary antibodies
  • Description
    Goat polyclonal to RENT1/hUPF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Drosophila melanogaster, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Immunogen was a synthetic peptide, to a region between residue 250 and 300 of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976).).

  • Positive control
    • Whole cell lysate from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2500. Detects a band of approximately 140 kDa (predicted molecular weight: 134 kDa).

Target

  • FunctionPlays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • DomainThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • Post-translational
    modifications
    Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

Anti-RENT1/hUPF1 antibody images



  • Predicted band size : 134 kDa


    ab10510 detection of human RENT1 by Western blot. Samples: Whole cell lysate (30 µg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: ab10510 used at 1 µg/ml. Detection: Chemiluminescence.

References for Anti-RENT1/hUPF1 antibody (ab10510)

This product has been referenced in:
  • Shi J  et al. Caffeine regulates alternative splicing in a subset of cancer-associated genes: a role for SC35. Mol Cell Biol 28:883-95 (2008). WB ; Human . Read more (PubMed: 18025108) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Arabidopsis thaliana Tissue lysate - whole (10 day old seedlings, grown on MS medium)
Gel Running Conditions Reduced Denaturing (8% gel)
Loading amount 100 µg
Specification 10 day old seedlings, grown on MS medium
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 22 2015

Thank you for contacting Abcam. Having looked at all our RENT1/UPF1 antibodies, I believe that ab10510 would be the most likely to work with your sample. Unfortunately I do not have access to the actual immunogen sequence that was used to gener...

Read More

Thank you for contacting us. We unfortunately do not have antibodies that are tested with Arabidopsis thaliana species. However after checking the immunogen sequence homology it seems that antibody ab10510 immunogen (amino acid 250 to 300) has a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Loading amount 50 µg
Specification U2OS cells
Gel Running Conditions Reduced Denaturing (4-20% tris-glycine)
Blocking step normal horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Natalie Farny

Verified customer

Submitted Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"