Overview

  • Product nameAnti-RENT1/hUPF1 antibody
    See all RENT1/hUPF1 primary antibodies
  • Description
    Mouse polyclonal to RENT1/hUPF1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 989-1088 of Human RENT1/hUPF1. Target sequence used to make the antibody: MPPMPPPDAG YFGQANGPAA GRGTPKGKTG RGGRQKNRFG LPGPSQTNLP NSQASQDVAS QPFSQGALTQ GYISMSQPSQ MSQPGLSQPE LSQDSYLGDE,

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 134 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionPlays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • DomainThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • Post-translational
    modifications
    Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP dependent helicase RENT1 antibody
    • ATP-dependent helicase RENT1 antibody
    • Delta helicase antibody
    • FLJ43809 antibody
    • FLJ46894 antibody
    • HUPF 1 antibody
    • hUpf1 antibody
    • KIAA0221 antibody
    • Nonsense mRNA reducing factor 1 antibody
    • NORF 1 antibody
    • NORF1 antibody
    • pNORF 1 antibody
    • pNORF1 antibody
    • Regulator of nonsense transcripts 1 antibody
    • RENT 1 antibody
    • RENT1 antibody
    • RENT1_HUMAN antibody
    • Smg 2 antibody
    • Smg 2 homolog nonsense mediated mRNA decay factor antibody
    • UP Frameshift 1 antibody
    • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
    • Up frameshift mutation 1 homolog antibody
    • Up frameshift suppressor 1 homolog antibody
    • Up-frameshift suppressor 1 homolog antibody
    • UPF 1 antibody
    • UPF 1 regulator of nonsense transcripts homolog antibody
    • upf1 antibody
    • UPF1 regulator of nonsense transcripts homolog antibody
    • UPF1 RNA helicase and ATPase antibody
    • Yeast Upf1p homolog antibody
    see all

Anti-RENT1/hUPF1 antibody images

  • All lanes : Anti-RENT1/hUPF1 antibody (ab43408) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a Tagged fusion protein of an irrelevant antigen
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) HRP conjugated, at 1/5000 dilution

    Predicted band size : 134 kDa

References for Anti-RENT1/hUPF1 antibody (ab43408)

ab43408 has not yet been referenced specifically in any publications.

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