The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
Use at 2-5 µg/mg of lysate.
Plays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
Belongs to the DNA2/NAM7 helicase family. Contains 1 C2H2-type zinc finger.
The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
Detection of RENT1/hUPF1 by Western Blot of Immunprecipitate.
ab86057 at 1µg/ml staining RENT1/hUPF1 in HeLa whole cell lysate immunoprecipitated using ab86057 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.