Anti-RENT1/hUPF1 antibody (ab92655)


  • Product nameAnti-RENT1/hUPF1 antibody
    See all RENT1/hUPF1 primary antibodies
  • Description
    Rabbit polyclonal to RENT1/hUPF1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from the internal region of Human RENT1/hUPF1.

  • Positive control
    • K562 cells, NIH/3T3 cells and Human colon carcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab92655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/20000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 124 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the DNA2/NAM7 helicase family.
      Contains 1 C2H2-type zinc finger.
    • DomainThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
    • Post-translational
      Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent helicase RENT1 antibody
      • ATP-dependent helicase RENT1 antibody
      • Delta helicase antibody
      • FLJ43809 antibody
      • FLJ46894 antibody
      • HUPF 1 antibody
      • hUpf1 antibody
      • KIAA0221 antibody
      • Nonsense mRNA reducing factor 1 antibody
      • NORF 1 antibody
      • NORF1 antibody
      • pNORF 1 antibody
      • pNORF1 antibody
      • Regulator of nonsense transcripts 1 antibody
      • RENT 1 antibody
      • RENT1 antibody
      • RENT1_HUMAN antibody
      • Smg 2 antibody
      • Smg 2 homolog nonsense mediated mRNA decay factor antibody
      • UP Frameshift 1 antibody
      • Up frameshift mutation 1 homolog (S. cerevisiae) antibody
      • Up frameshift mutation 1 homolog antibody
      • Up frameshift suppressor 1 homolog antibody
      • Up-frameshift suppressor 1 homolog antibody
      • UPF 1 antibody
      • UPF 1 regulator of nonsense transcripts homolog antibody
      • upf1 antibody
      • UPF1 regulator of nonsense transcripts homolog antibody
      • UPF1 RNA helicase and ATPase antibody
      • Yeast Upf1p homolog antibody
      see all

    Anti-RENT1/hUPF1 antibody images

    • All lanes : Anti-RENT1/hUPF1 antibody (ab92655) at 1/500 dilution

      Lane 1 : Extract from NIH/3T3 cells (without competing peptide)
      Lane 2 : Extract from K562 cells (without competing peptide)
      Lane 3 : Extract from NIH/3T3 cells (with competing peptide)

      Lysates/proteins at 5 µg per lane.

      Predicted band size : 124 kDa
    • ab92655, at 1/50 dilution ,staining RENT1/hUPF1 in paraffin-embedded Human colon carcinoma tissue (1) without competing peptide and (2) with competing peptide, by Immunohistochemistry (Formalin/PFA-fixed parafin-embedded sections).

    References for Anti-RENT1/hUPF1 antibody (ab92655)

    ab92655 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam about ab109363, ab92655 and ab10534. Unfortunately the immunogen sequences for these three antibodies is proprietary information and I do not have access to that information. Please except my apologies for n...

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