Anti-Reptin/TIP49B/RUVB2 antibody (ab137834)
Key features and details
- Rabbit polyclonal to Reptin/TIP49B/RUVB2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Reptin/TIP49B/RUVB2 antibody
See all Reptin/TIP49B/RUVB2 primary antibodies -
Description
Rabbit polyclonal to Reptin/TIP49B/RUVB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-303 of Human Reptin/TIP49B/RUVB2 (UniProt ID: Q9Y230).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt4 and Raji whole cell lysates; A549 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2. -
Tissue specificity
Ubiquitously expressed. Highly expressed in testis and thymus. -
Sequence similarities
Belongs to the ruvB family. -
Domain
The C-terminal domain is required for association with ATF2. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes. - Information by UniProt
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Database links
- Entrez Gene: 511048 Cow
- Entrez Gene: 10856 Human
- Entrez Gene: 20174 Mouse
- Entrez Gene: 292907 Rat
- Entrez Gene: 380092 Xenopus laevis
- Entrez Gene: 317678 Zebrafish
- Omim: 604788 Human
- SwissProt: Q2TBU9 Cow
see all -
Alternative names
- 48 kDa TATA box-binding protein-interacting protein antibody
- 48 kDa TBP-interacting protein antibody
- 48-kDa TATA box-binding protein-interacting protein antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab137834 has been referenced in 1 publication.
- Raymond AA et al. Reptin regulates DNA double strand breaks repair in human hepatocellular carcinoma. PLoS One 10:e0123333 (2015). WB ; Human . PubMed: 25875766