Anti-Reptin/TIP49B/RUVB2 antibody (ab89942)

Overview

  • Product nameAnti-Reptin/TIP49B/RUVB2 antibody
    See all Reptin/TIP49B/RUVB2 primary antibodies
  • Description
    Mouse polyclonal to Reptin/TIP49B/RUVB2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Reptin/TIP49B/RUVB2 (NP_006657.1, amino acids 1-463).

  • Positive control
    • Human pancreas tissue lysate, HeLa cell lysate, Reptin/TIP49B/RUVB2 transfected 293T cell lysate, HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab89942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionPossesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
  • Tissue specificityUbiquitously expressed. Highly expressed in testis and thymus.
  • Sequence similaritiesBelongs to the ruvB family.
  • DomainThe C-terminal domain is required for association with ATF2.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 48 kDa TATA box-binding protein-interacting protein antibody
    • 48 kDa TBP-interacting protein antibody
    • 48-kDa TATA box-binding protein-interacting protein antibody
    • 48-kDa TBP-interacting protein antibody
    • 51 kDa erythrocyte cytosolic protein antibody
    • CGI-46 antibody
    • EC=3.6.1.- antibody
    • ECP-51 antibody
    • ECP51 antibody
    • Erythrocyte cytosolic protein, 51-KD antibody
    • INO80 complex subunit J antibody
    • INO80J antibody
    • MGC144733 antibody
    • MGC144734 antibody
    • MGC52995 antibody
    • mp47 antibody
    • p47 antibody
    • p47 protein antibody
    • Repressing pontin 52 antibody
    • Reptin 52 antibody
    • REPTIN antibody
    • RuvB (E coli homolog)-like 2 antibody
    • RUVB, E. coli, homolog-like 2 antibody
    • RuvB-like 2 (E. coli) antibody
    • RuvB-like 2 antibody
    • RuvB-like protein 2 antibody
    • RUVB2 antibody
    • RUVB2_HUMAN antibody
    • RUVBL2 antibody
    • RVB2 antibody
    • TAP54-beta antibody
    • TATA box-binding protein-interacting protein, 48-KD antibody
    • TBP-interacting protein, 48-KD antibody
    • TIH2 antibody
    • TIP48 antibody
    • TIP49b antibody
    • TIP60-associated protein 54-beta antibody
    • wu:fi25f01 antibody
    • zreptin antibody
    see all

Anti-Reptin/TIP49B/RUVB2 antibody images

  • Anti-Reptin/TIP49B/RUVB2 antibody (ab89942) at 1 µg/ml + Human pancreas tissue lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • Anti-Reptin/TIP49B/RUVB2 antibody (ab89942) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab89942) at 1 µg/ml

    Lane 1 : Reptin/TIP49B/RUVB2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • ab89942 at 10 µg/ml staining Reptin/TIP49B/RUVB2 in HeLa cells by Immunofluorescence.

References for Anti-Reptin/TIP49B/RUVB2 antibody (ab89942)

ab89942 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Cell lysate - whole cell (load 1 embryo @ 1dpf per lane)
Loading amount 1 µg
Specification load 1 embryo @ 1dpf per lane
Treatment overexpressed with GFP-Reptin
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 07 2010

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