The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 5-15 µg/mg of lysate.
Is unsuitable for WB.
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
Ubiquitously expressed. Highly expressed in testis and thymus.
Belongs to the ruvB family.
The C-terminal domain is required for association with ATF2.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
ab91461 at 1 µg/ml detecting Reptin/TIP49B/RUVB2 in HeLa whole cell lysate by WB following IP. Immunoprecipitation utilised ab91461 at 10 µg/mg of lysate (lane 2). Reptin/TIP49B/RUVB2 was also immunoprecipitated by an antibody to an upstream epitope of Reptin/TIP49B/RUVB2 (lane 1) but not by control IgG (lane 3). 1 mg of lysate was used for IP and 20% of IP was loaded.
Detection utilised ECL with a 30 second exposure.