Anti-Reptin/TIP49B/RUVB2 antibody (ab91462)


  • Product nameAnti-Reptin/TIP49B/RUVB2 antibody
    See all Reptin/TIP49B/RUVB2 primary antibodies
  • Description
    Rabbit polyclonal to Reptin/TIP49B/RUVB2
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human, Zebrafish
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-50 (MATVTATTKV PEIRDVTRIE RIGAHSHIRG LGLDDALEPR QASQGMVGQL) of Human Reptin/TIP49B/RUVB2 (NP_006657.1).

  • Positive control
    • Whole cell lystae from HeLa cells, 293T cells and Mouse NIH3T3 cells.



Our Abpromise guarantee covers the use of ab91462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 51 kDa.
IP Use at 5-15 µg/mg of lysate.


  • FunctionPossesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex. May also inhibit the transcriptional activity of ATF2.
  • Tissue specificityUbiquitously expressed. Highly expressed in testis and thymus.
  • Sequence similaritiesBelongs to the ruvB family.
  • DomainThe C-terminal domain is required for association with ATF2.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol. Although it is also present in the cytoplasm and associated with the cell membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 48 kDa TATA box-binding protein-interacting protein antibody
    • 48 kDa TBP-interacting protein antibody
    • 48-kDa TATA box-binding protein-interacting protein antibody
    • 48-kDa TBP-interacting protein antibody
    • 51 kDa erythrocyte cytosolic protein antibody
    • CGI-46 antibody
    • EC=3.6.1.- antibody
    • ECP-51 antibody
    • ECP51 antibody
    • Erythrocyte cytosolic protein, 51-KD antibody
    • INO80 complex subunit J antibody
    • INO80J antibody
    • MGC144733 antibody
    • MGC144734 antibody
    • MGC52995 antibody
    • mp47 antibody
    • p47 antibody
    • p47 protein antibody
    • Repressing pontin 52 antibody
    • Reptin 52 antibody
    • REPTIN antibody
    • RuvB (E coli homolog)-like 2 antibody
    • RUVB, E. coli, homolog-like 2 antibody
    • RuvB-like 2 (E. coli) antibody
    • RuvB-like 2 antibody
    • RuvB-like protein 2 antibody
    • RUVB2 antibody
    • RUVB2_HUMAN antibody
    • RUVBL2 antibody
    • RVB2 antibody
    • TAP54-beta antibody
    • TATA box-binding protein-interacting protein, 48-KD antibody
    • TBP-interacting protein, 48-KD antibody
    • TIH2 antibody
    • TIP48 antibody
    • TIP49b antibody
    • TIP60-associated protein 54-beta antibody
    • wu:fi25f01 antibody
    • zreptin antibody
    see all

Anti-Reptin/TIP49B/RUVB2 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Reptin/TIP49B/RUVB2 with ab91462 at 1/1000 (1µg/ml). Detection: DAB.
  • ab91462 at 1 µg/ml detecting Reptin/TIP49B/RUVB2 in HeLa whole cell lysate by WB following IP. Immunoprecipitation utilised ab91462 at 10 µg/mg of lysate (lane 1). Reptin/TIP49B/RUVB2 was also immunoprecipitated by an antibody to an downstream epitope of Reptin/TIP49B/RUVB2 (lane 2) but not by control IgG (lane 3). 1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 30 second exposure.
  • All lanes : Anti-Reptin/TIP49B/RUVB2 antibody (ab91462) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 51 kDa

    Exposure time : 3 minutes

References for Anti-Reptin/TIP49B/RUVB2 antibody (ab91462)

ab91462 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (load 1 embryo @ 4dpf)
Loading amount 1 µg
Specification load 1 embryo @ 4dpf
Treatment inject GFP-Reptin mRNA @ 1 cell stage
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Sep 23 2010