Anti-Repulsive Guidance Molecule A antibody (ab26287)

Overview

  • Product name
    Anti-Repulsive Guidance Molecule A antibody
    See all Repulsive Guidance Molecule A primary antibodies
  • Description
    Rabbit polyclonal to Repulsive Guidance Molecule A
  • Tested applications
    Suitable for: IHC-P, IHC-FoFr, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Mouse Repulsive Guidance Molecule A.

    (Peptide available as ab27889.)

  • Positive control
    • Rat Brain Whole Tissue Lysate, Mouse Brain Whole Tissue Lysate, Rat Spinal Cord Tissue Lysate and Mouse Spinal Cord Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab26287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 21467223

Target

  • Function
    Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.
  • Sequence similarities
    Belongs to the repulsive guidance molecule (RGM) family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Repulsive guidance molecule A antibody
    • RGM antibody
    • RGM domain family member A antibody
    • RGMA antibody
    • RGMA_HUMAN antibody
    see all

Images

  • All lanes : Anti-Repulsive Guidance Molecule A antibody (ab26287) at 1 µg/ml

    Lane 1 : Rat Brain Whole Tissue Lysate
    Lane 2 : Mouse Brain Whole Tissue Lysate
    Lane 3 : Rat Spinal Cord Tissue Lysate
    Lane 4 : Mouse Spinal Cord Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • All lanes : Anti-Repulsive Guidance Molecule A antibody (ab26287) at 1/1000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse Spinal Cord
    Lane 3 : Mouse embryonic muscle
    Lane 4 : Mouse embronic brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    Dako cytomation Conjugation: HRP at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 18 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

  • Immunohistochemistical (Formalin/PFA-fixed paraffin-embedded sections) detection of Repulsive Guidance Molecule A antibody (ab26287) in mouse brain. Fixative used: Formaldehyde; Antigen retrieval step: None;  Permeabilization: Yes; Blocking step: 1% Serum for 1 hour @ 25°C. Primary Antibody used at 1/500 incubated for 24 h @ 4°C in 0.1% PBS+ 0.3 Triton X+ 1% goat serum.  This image shows RGM-A expressed in all six layers of cortical neurons of adult mouse brain.

    See Abreview

  • IHC-FoFr image of Repulsive Guidance Molecule A staining in rat cortex (left) and hippocampus (right) using ab26287. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight.

    See Abreview

  • IHC image of Repulsive Guidance Molecule A staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab26287, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References

This product has been referenced in:
  • Xu X  et al. Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS. Hum Vaccin Immunother 12:2169-2180 (2016). ELISA ; Mouse . Read more (PubMed: 26986456) »
  • Mirakaj V  et al. Repulsive guidance molecule-A (RGM-A) inhibits leukocyte migration and mitigates inflammation. Proc Natl Acad Sci U S A 108:6555-60 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21467223) »

See all 3 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Username

Dr. Sophie Pezet

Verified customer

Submitted Nov 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Fixative
Formaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% PBS+ 0.3% Triton X 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 08 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, spinal cord)
Loading amount
20 µg
Specification
Brain, spinal cord
Gel Running Conditions
Reduced Denaturing (10% SDS/PAGE)
Blocking step
No blocking step used for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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