Anti-Repulsive Guidance Molecule C antibody [Fery-1] (ab54431)

Overview

  • Product nameAnti-Repulsive Guidance Molecule C antibody [Fery-1]
    See all Repulsive Guidance Molecule C primary antibodies
  • Description
    Mouse monoclonal [Fery-1] to Repulsive Guidance Molecule C
  • Specificityab54431 recognizes human Repulsive Guidance Molecule C.
  • Tested applicationsSuitable for: IP, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 36-398 of Human Repulsive Guidance Molecule C

Properties

Applications

Our Abpromise guarantee covers the use of ab54431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 50, 37, 45, 74, 100 kDa (predicted molecular weight: 50, 37 kDa).

Target

  • RelevanceMutations in Repulsive Guidance Molecule (also known as RGM-C; HJV; hemojuvelin; Hfe2) cause juvenile hemochromatosis, a severe iron overload disease. RGM-C gene expression has been characterized in the developing mouse and found to be exclusively expressed in all striated muscle and in the myocardium.
  • Cellular localizationCell membrane; Lipid-anchor, GPI-anchor
  • Database links
  • Alternative names
    • 2310035L15Rik antibody
    • 5230400G09Rik antibody
    • AI414844 antibody
    • AI789733 antibody
    • DL M antibody
    • Haemojuvelin antibody
    • HEMOCHROMATOSIS antibody
    • HEMOCHROMATOSIS DUE TO DEFECT IN HEMOJUVELIN antibody
    • HEMOCHROMATOSIS DUE TO DEFECT IN HEPCIDIN ANTIMICROBIAL PEPTIDE antibody
    • HEMOCHROMATOSIS JUVENILE antibody
    • Hemochromatosis type 2 (juvenile) antibody
    • Hemochromatosis type 2 antibody
    • Hemochromatosis type 2 protein antibody
    • Hemochromatosis type 2 protein homolog antibody
    • HEMOCHROMATOSIS, TYPE 2A antibody
    • HEMOCHROMATOSIS, TYPE 2B antibody
    • Hemojuvelin antibody
    • HFE 2 antibody
    • Hfe2 antibody
    • HFE2A antibody
    • HJV antibody
    • JH antibody
    • Juvenile antibody
    • MGC23953 antibody
    • OTTHUMP00000059680 antibody
    • Repulsive guidance molecule c antibody
    • RGM C antibody
    • RGM domain family member C antibody
    • RGMC antibody
    see all

Anti-Repulsive Guidance Molecule C antibody [Fery-1] images

  • All lanes : Anti-Repulsive Guidance Molecule C antibody [Fery-1] (ab54431) at 1/1000 dilution

    Lane 1 : embryonic day 16 mouse muscle
    Lane 2 : adult mouse heart

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP conjugated at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50, 37 kDa
    Additional bands at : 45&74&100 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

    See Abreview

References for Anti-Repulsive Guidance Molecule C antibody [Fery-1] (ab54431)

This product has been referenced in:
  • van Swelm RP  et al. Acute acetaminophen intoxication leads to hepatic iron loading by decreased hepcidin synthesis. Toxicol Sci 129:225-33 (2012). Read more (PubMed: 22610607) »
  • Lakhal S  et al. Regulation of Type II Transmembrane Serine Proteinase TMPRSS6 by Hypoxia-inducible Factors: NEW LINK BETWEEN HYPOXIA SIGNALING AND IRON HOMEOSTASIS. J Biol Chem 286:4090-7 (2011). Flow Cyt ; Human . Read more (PubMed: 20966077) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 11 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Muscle)
Loading amount 20 µg
Specification Muscle
Gel Running Conditions Reduced Denaturing (12% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Mar 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"