Overview

  • Product name
  • Description
    Rabbit polyclonal to RERE
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within amino acids 389-418 from the N terminal region of Human RERE.

  • Positive control
    • Neuro-2a cell line lysates; lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab103462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 172 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Plays a role as a transcriptional repressor during development. May play a role in the control of cell survival. Overexpression of RERE recruits BAX to the nucleus particularly to POD and triggers caspase-3 activation, leading to cell death.
  • Tissue specificity
    Widely expressed. Expressed in tumor cell lines.
  • Involvement in disease
    Note=A chromosomal aberration involving RERE is found in the neuroblastoma cell line. Translocation t(1;15)(p36.2;q24).
  • Sequence similarities
    Contains 1 BAH domain.
    Contains 1 ELM2 domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 SANT domain.
  • Domain
    The interaction with ATN1 is mediated by the coiled coil domain.
  • Cellular localization
    Nucleus. Localized in nuclear bodies of variables size. Colocalized with PML and BAX in nuclear PODs.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110033A15Rik antibody
    • AI414665 antibody
    • ARG antibody
    • arginine glutamic acid dipeptide (RE) repeats antibody
    • arginine glutamic acid dipeptide repeats antibody
    • Arginine glutamic acid dipeptide repeats protein antibody
    • Arginine-glutamic acid dipeptide repeats protein antibody
    • ARGININE-GLUTAMIC ACID REPEATS-ENCODING GENE antibody
    • ARP antibody
    • ATN1L antibody
    • Atr2 antibody
    • atrophin 1 like antibody
    • Atrophin 1 like protein antibody
    • Atrophin 1 related protein antibody
    • atrophin 2 antibody
    • atrophin related protein antibody
    • ATROPHIN-1-ASSOCIATED PROTEIN antibody
    • Atrophin-1-like protein antibody
    • Atrophin-1-related protein antibody
    • atrophin-2 antibody
    • ATROPHIN-RELATED PROTEIN antibody
    • AW742570 antibody
    • DNB1 antibody
    • FLJ38775 antibody
    • KIAA0458 antibody
    • mKIAA0458 antibody
    • NEDBEH antibody
    • OTTHUMP00000001701 antibody
    • Rere antibody
    • RERE_HUMAN antibody
    see all

Images

  • Anti-RERE antibody (ab103462) at 1/100 dilution + Neuro 2a cell line lysate at 35 µg

    Predicted band size : 172 kDa
  • ab103462, at a 1/50 dilution, staining RERE in formalin fixed and paraffin embedded lung carcinoma tissue by Immunohistochemistry. Developed using peroxidase conjugated secondary antibody and DAB staining.

References

ab103462 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA-Buffer pH 9,0
Permeabilization
Yes - Wash-Buffer from DAKO with Tween
Username

Mr. Rudolf Jung

Verified customer

Submitted Feb 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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