RERG overexpression 293T lysate (whole cell) (ab94205)

Overview

  • Product nameRERG overexpression 293T lysate (whole cell)
  • Description
    RERG overexpression lysate
  • General notesab94205 is a 293T cell transfected lysate in which Human RERG has been transiently over-expressed using a pCMV-RERG plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Binds GDP/GTP and possesses intrinsic GTPase activity. Has higher affinity for GDP than for GTP. In cell lines overexpression leads to a reduction in the rate of proliferation, colony formation and in tumorigenic potential. Tissue specificity: Detected in heart, brain, placenta, lung, liver, skin, kidney and pancreas. Detected in estrogen receptor-positive breast-derived cell lines, but not in estrogen receptor-negative cell lines. Expression is decreased or lost in a significant proportion of primary breast tumors with poor clinical prognosis. Similarity: Belongs to the small GTPase superfamily. Ras family.

Applications

Our Abpromise guarantee covers the use of ab94205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

RERG overexpression 293T lysate (whole cell) images

  • ab94205 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-RERG antibody (ab67164) at 1/500 dilution

    Lane 1 : RERG overexpression 293T lysate (whole cell) (ab94205)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for RERG overexpression 293T lysate (whole cell) (ab94205)

ab94205 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"