All tags HIF-1a Hypoxia markers guide

Hypoxia markers guide

Find the products you need to detect hypoxia using protein markers such as HIF-1a and BNIP3.

HIF-1α

Under hypoxia, HIF-1a is stabilized and moves into the nucleus, where it regulates transcription of genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia.

Figure 1. HIF-1a staining of HeLa cells. Anti-HIF-1a antibody [EPR16897] (ab179483) was used at 1/50 dilution with goat anti-rabbit IgG (Alexa Fluor® 488) secondary antibody. Cells were treated with 0.6 nM CoCl2 for 6 hours.


ApplicationRecommended product
Western blot, immunocytochemistryAnti-HIF-1a antibody (ab179483)
ELISAHuman SimpleStep ELISA® kit (ab171577)
Positive controlRecombinant human HIF-1a protein (ab154478)
Activity assayHIF-1a transcription factor assay kit (ab133104)

See all HIF-1a products.


BNIP3

BNIP3 induction by HIF-1α under hypoxic conditions is involved in hypoxia-mediated autophagy, cell survival and tumor progression1.

Figure 2. BNIP3 staining of laryngeal squamous cell carcinoma tissue. Anti-BNIP3 mouse monoclonal antibody (ab10433) was used at 1/100 dilution and visualized by DAB.


ApplicationRecommended product
VariousAnti-BNIP antibody [ANa40] (ab10433)
ELISAHuman HIF-1a + BNIP3 hypoxia in cell ELISA kit (ab129733)
Flow cytometryHIF-1a + BNIP3 hypoxia response human flow cytometry kit (ab126585)

See all BNIP3 products.


PDK1

PDK1 (pyruvate dehydrogenase kinase 1) is downstream of HIF-1α and is induced under hypoxia. Expression during hypoxia channels glucose through glycolysis to maintain ATP production2.

Figure 3. PDK1 staining of NIH/3T3 cells. Anti-mitochondrial pyruvate dehydrogenase kinase 1 antibody (ab202468) was used at a dilution of 1/1000 with goat anti-rabbit IgG (Alexa Fluor 488) secondary antibody (ab150077). Cells were counterstained with DAPI (blue) and  tubulin also stained (red).

ApplicationRecommended product
Western blot, ICC, IP, flow cytometryAnti-mitochondrial pyruvate dehydrogenase kinase 1 antibody (ab202468)
ELISAHuman HIF-1a + PDK1 hypoxia in cell ELISA kit (ab125299)
Flow cytometryHIF-1a + PDK1 hypoxia response human flow cytometry kit (ab126700)
Positive controlRecombinant human mitochondrial pyruvate dehydrogenase kinase 1 protein (ab125580)

See all PDK1 products.


GLUT1

Expression of the GLUT1 glucose transporter is increased under hypoxic conditions, induced by HIF-1α3.

Figure 4. GLUT1 staining of HEPg2 cells. Anti-glucose transporter GLUT antibody (ab115730) was used at a dilution of 1/100 with Alexa Fluor® 488 goat anti-rabbit secondary antibody (ab150077). Cells were counterstained with DAPI and tubulin was also stained (red).


ApplicationRecommended product
Western blot, ICC/IHC, flow cytometryAnti-glucose transporter GLUT antibody (ab115730)
ELISAHuman HIF-1a + GLUT hypoxia in cell ELISA kit (ab125298)
Flow cytometryHIF-1a + GLUT1 hypoxia response human flow cytometry kit (ab126584)

See all GLUT1 products.



References

1. Bellot G, Garcia-Medina, Gounon P, Chiche J, Roux D, Pouysségur J, Mazure NM. Hypoxia-induced autophagy is mediated through hypoxia-inducible factor induction of BNIP3 and BNIP3L via their BH3 domains. Mol Cell Biol. 10, 2570–81 (2009).

2. Kim JW, Tchernyshyov I, Semenza GL, Dang CV. HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxia. Cell Metab. 3, 177–85 (2006).

3. Chen C, Pore N, Behrooz A, Ismail-Beigi F, Maity A. Regulation of glut1 mRNA by hypoxia-inducible factor-1. Interaction between H-ras and hypoxia. J Biol Chem. 276, 9519–25 (2000).




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