Anti-Respiratory Syncytial Virus antibody [2F7] (ab43812)
- Datasheet
- Specific References (10)
- Protocols
Overview
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Product nameAnti-Respiratory Syncytial Virus antibody [2F7]
See all Respiratory Syncytial Virus primary antibodies -
DescriptionMouse monoclonal [2F7] to Respiratory Syncytial Virus
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Host speciesMouse
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Tested applicationsSuitable for: WB, IHC-Fr, ICC, ELISAmore details
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Species reactivityRespiratory Syncytial Virus (RSV) strains (Paramyxovirus 1, 2 & 3). No cross-reactivity with other respiratory viruses.
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Immunogen
Recombinant FP
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number2F7
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab43812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/5000. Predicted molecular weight: 48 , 70 kDa. | |
| IHC-Fr | 1/100 - 1/500. | |
| ICC | 1/100 - 1/500. Immunocytology on infected cells. |
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| ELISA | 1/2000 - 1/1000. |
Target
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RelevanceRespiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.
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Cellular localizationVirion. Host cytoplasm
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Alternative names
- RS virus antibody
- RSV antibody
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial Virus antibody [2F7] (ab43812)](http://a.abcam.com/ps/products/43/ab43812/Images/ab43812-264031-43812-ICCIF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial Virus antibody [2F7] (ab43812)Image from Krzyzaniak MA et al., PLoS Pathogens. 2013;9(4):e1003309. Fig 2.; doi: 10.1371/journal.ppat.1003309Immunofluorescent staining of Respiratory Syncytial Virus F protein Bound to HeLa cells using ab43812. Respiratory Syncytial Virus F protein was visualized in green and Respiratory Syncytial N protein in red.
Protocols
References
This product has been referenced in:
- Mehedi M et al. Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells. Bio Protoc 7:N/A (2017). Read more (PubMed: 29057295) »
- Liang B et al. Improved Prefusion Stability, Optimized Codon Usage, and Augmented Virion Packaging Enhance the Immunogenicity of Respiratory Syncytial Virus Fusion Protein in a Vectored-Vaccine Candidate. J Virol 91:N/A (2017). Read more (PubMed: 28539444) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"