Purification notesSodium Sulfate precipitation and ion exchange chromatography. Covalently coupled with highly purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 20647383
Use at an assay dependent concentration.
1/10 - 1/50.
RelevanceRespiratory syncytial virus (RSV) is a major cause of respiratory illness in young children. RSV infection produces a variety of signs and symptoms involving different areas of the respiratory tract, from the nose to the lungs. RSV is a negative sense, enveloped RNA virus. The virion is variable in shape and size with average diameter of between 120 and 300 nm. The 63 kD RSV fusion protein of the RSS 2 strain (subtype A) directs fusion of viral and cellular membranes, results in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia or multi nucleated giant cells.
Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial Virus antibody (FITC) (ab20391)Image from Munday DC et al., Mol Cell Proteomics. 2010 Nov;9(11):2438-59. Epub 2010 Jul 20. Fig 12.; doi: 10.1074/mcp.M110.001859; November 1, 2010, Molecular & Cellular Proteomics, 9, 2438-2459.
Immunofluorescence analysis of HRSV-infected A549 cells, staining Respiratory Syncytial Virus with ab20391. The cells were fixed with formalin and detected with primary antibody.
References for Anti-Respiratory Syncytial Virus antibody (FITC) (ab20391)
This product has been referenced in:
Wu W et al. Characterization of the interaction between human respiratory syncytial virus and the cell cycle in continuous cell culture and primary human airway epithelial cells. J Virol85:10300-9 (2011).
Read more (PubMed: 21795354) »
Munday DC et al. Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus. Mol Cell Proteomics9:2438-59 (2010).
Read more (PubMed: 20647383) »