Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein antibody [RSV5A6] (ab94968)
Key features and details
- Mouse monoclonal [RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein
- Suitable for: ELISA, WB, ICC/IF, IHC-P, IHC-Fr
- Reacts with: Respiratory syncytial virus
- Isotype: IgG2a
Overview
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Product name
Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein antibody [RSV5A6]
See all Respiratory Syncytial Virus Fusion (F) Glycoprotein primary antibodies -
Description
Mouse monoclonal [RSV5A6] to Respiratory Syncytial Virus Fusion (F) Glycoprotein -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WB, ICC/IF, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Respiratory syncytial virus -
Immunogen
Tissue, cells or virus corresponding to Respiratory Syncytial Virus Fusion (F) Glycoprotein. hRSV strain A2 infected HeLa cells
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General notes
Fusion partner: PS-NS/1-Ag4ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Primary antibody notes
ab94968 is useful for the identification and location of expression of the fusion glycoprotein of Human Respiratory Syncytial Virus of both sub-groups A and B. -
Clonality
Monoclonal -
Clone number
RSV5A6 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
Fix with Acetone. |
Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. Fix with Acetone. |
Target
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Relevance
Respiratory Syncytial Virus (RSV) Fusion (F) Glycoprotein is a Class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Directs fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm. This fusion is pH independent and occurs directly at the outer cell membrane. The trimer of F1-F2 (protein F) interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and interacts with the cellular membrane, inducing the fusion between host cell and virion membranes. Notably, RSV fusion protein is able to interact directly with heparan sulfate and therefore actively participates in virus attachment. Furthermore, the F2 subunit was identifed as the major determinant of RSV host cell specificity. Later in infection, proteins F expressed at the plasma membrane of infected cells mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis. The fusion protein is also able to trigger p53-dependent apoptosis. -
Cellular localization
Virion membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass membrane protein. -
Database links
- SwissProt: P03420 Respiratory syncytial virus
- SwissProt: P12568 Respiratory syncytial virus
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Alternative names
- F antibody
- Fusion glycoprotein F0 antibody
- Protein F antibody
- RSV Fusion (F) Glycoprotein antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab94968 has been referenced in 7 publications.
- Niyomdecha N et al. Repurposing of antiparasitic niclosamide to inhibit respiratory syncytial virus (RSV) replication. Virus Res 295:198277 (2021). PubMed: 33476693
- Tang W et al. Mechanism of Cross-Resistance to Fusion Inhibitors Conferred by the K394R Mutation in Respiratory Syncytial Virus Fusion Protein. J Virol 95:e0120521 (2021). PubMed: 34379500
- Touzelet O et al. The Secretome Profiling of a Pediatric Airway Epithelium Infected with hRSV Identified Aberrant Apical/Basolateral Trafficking and Novel Immune Modulating (CXCL6, CXCL16, CSF3) and Antiviral (CEACAM1) Proteins. Mol Cell Proteomics 19:793-807 (2020). PubMed: 32075873
- Li J et al. Long Noncoding RNA NRAV Promotes Respiratory Syncytial Virus Replication by Targeting the MicroRNA miR-509-3p/Rab5c Axis To Regulate Vesicle Transportation. J Virol 94:N/A (2020). PubMed: 32102886
- Li M et al. Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. J Virol 92:N/A (2018). PubMed: 29386287
- Vanover D et al. RSV glycoprotein and genomic RNA dynamics reveal filament assembly prior to the plasma membrane. Nat Commun 8:667 (2017). PubMed: 28939853
- West WH et al. Biological activity, binding site and affinity of monoclonal antibodies to the fusion protein of respiratory syncytial virus. J Gen Virol 75 ( Pt 10):2813-9 (1994). PubMed: 7931172