Anti-Respiratory Syncytial Virus fusion protein antibody [RSV3216 (B016)] (ab24011)

Overview

  • Product name
    Anti-Respiratory Syncytial Virus fusion protein antibody [RSV3216 (B016)]
  • Description
    Mouse monoclonal [RSV3216 (B016)] to Respiratory Syncytial Virus fusion protein
  • Specificity
    Recognises a Respiratory Syncytial Virus fusion protein (46kD and 22kD s-s linked glycoprotein).
  • Tested applications
    Suitable for: Flow Cyt, WB, IP, ICC/IF, Electron Microscopy, Sandwich ELISAmore details
  • Species reactivity
    Cross-reacts with Respiratory Syncytial Virus.
  • Immunogen

    Bovine RSV strains, 127, SNK and 9007. Human RSV strains; Long, Randall, 8/60, and A/2.

Properties

Applications

Our Abpromise guarantee covers the use of ab24011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [B023] to Respiratory Syncytial Virus Nucleoprotein (ab22501). Use ab34992 as capture antibody

Target

  • Relevance
    Respiratory Syncytial Virus fusion protein directs fusion of viral and cellular membranes, resulting in viral penetration, and can direct fusion of infected cells with adjoining cells, resulting in the formation of syncytia.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • Fusion glycoprotein F0 antibody
    • Fusion glycoprotein F1 antibody
    • Fusion glycoprotein F2 antibody
    • Fusion protein (F) antibody
    • RSV fusion protein antibody
    see all

References

This product has been referenced in:
  • Jans J  et al. Actin- and clathrin-dependent mechanisms regulate interferon gamma release after stimulation of human immune cells with respiratory syncytial virus. Virol J 13:52 (2016). Read more (PubMed: 27004689) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
Fix/Perm
Permeabilization
Yes - Perm/Wash (BD Biosciences)
Sample
Human Cell (CD14+ PBMC infected with RSV)
Specification
CD14+ PBMC infected with RSV
Gating Strategy
PBMCs were gated on CD14
Preparation
Cell harvesting/tissue preparation method: Ficoll isolated PBMCs infected with RSV with moi of 1
Sample buffer: CytoFix/CytoPerm (BD Biosciences)
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Submitted Aug 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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