Overview

  • Product nameAnti-REST / NRSF antibody
    See all REST / NRSF primary antibodies
  • Description
    Rabbit polyclonal to REST / NRSF
  • Tested applicationsSuitable for: IHC-FoFr, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1050 to the C-terminus of Mouse REST/ NRSF.

    (Peptide available as ab17042.)

  • Positive control
    • This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab21635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 21715642Fixed for 10 min in cold methanol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionTranscriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
    • Tissue specificityUbiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
    • Sequence similaritiesContains 9 C2H2-type zinc fingers.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Neural Restrictive Silencer Factor antibody
      • Neural-restrictive silencer factor antibody
      • Neuron Restrictive Silencer Factor antibody
      • NRSF antibody
      • RE1 silencing transcription factor antibody
      • RE1-silencing transcription factor antibody
      • Repressor binding to the X2 box antibody
      • rest antibody
      • REST protein antibody
      • REST_HUMAN antibody
      • X2 box repressor antibody
      • XBR antibody
      see all

    Anti-REST / NRSF antibody images

    • All lanes : Anti-REST / NRSF antibody (ab21635) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/ml
      Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Mouse REST / NRSF peptide (ab17042) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 121 kDa
      Observed band size : 121 kDa
      Additional bands at : 53 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    References for Anti-REST / NRSF antibody (ab21635)

    This product has been referenced in:
    • Cox EJ & Marsh SA Exercise and diabetes have opposite effects on the assembly and O-GlcNAc modification of the mSin3A/HDAC1/2 complex in the heart. Cardiovasc Diabetol 12:101 (2013). Read more (PubMed: 23835259) »
    • Mapp OM  et al. Zebrafish Prickle1b mediates facial branchiomotor neuron migration via a farnesylation-dependent nuclear activity. Development 138:2121-32 (2011). IHC (PFA fixed) ; Zebrafish . Read more (PubMed: 21521740) »

    See all 7 Publications for this product

    Product Wall

    Application Western blot
    Sample Rat Tissue lysate - nuclear (Hippocampus, liver and MCF7 whole cell homogenates)
    Gel Running Conditions Reduced Denaturing (10)
    Loading amount 80 µg
    Specification Hippocampus, liver and MCF7 whole cell homogenates
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Octavio Mercado-Gomez

    Verified customer

    Submitted May 12 2016

    Application Western blot
    Loading amount 90 µg
    Gel Running Conditions Reduced Denaturing (4-18% gel)
    Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Specification Mouse embryonic stem cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 17 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization Yes - 0.3% TRITON-X
    Specification Brain
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 14 2014

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. The details you have kindly provided will enable us to investigate this case f...

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    Thank you for contacting us. I have forwarded your data to our lab for analysis. It would be very helpful if you could please provide some additional details about your protocol: 1.) How were the tissues fixed and processed (paraffin or froze...

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