Overview

  • Product nameAnti-REST / NRSF antibody
    See all REST / NRSF primary antibodies
  • Description
    Rabbit polyclonal to REST / NRSF
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from within residues 250 - 350 of Human REST.

    (Peptide available as ab22400.)

  • Positive control
    • This antibody gave a positive signal in HeLa and HEK293 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab28018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionTranscriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
    • Tissue specificityUbiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
    • Sequence similaritiesContains 9 C2H2-type zinc fingers.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Neural Restrictive Silencer Factor antibody
      • Neural-restrictive silencer factor antibody
      • Neuron Restrictive Silencer Factor antibody
      • NRSF antibody
      • RE1 silencing transcription factor antibody
      • RE1-silencing transcription factor antibody
      • Repressor binding to the X2 box antibody
      • rest antibody
      • REST protein antibody
      • REST_HUMAN antibody
      • X2 box repressor antibody
      • XBR antibody
      see all

    Anti-REST / NRSF antibody images

    • All lanes : Anti-REST / NRSF antibody (ab28018) at 1 µg/ml

      Lane 1 : HEK293 Lysate
      Lane 2 : Hela Lysate

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 121 kDa
      Observed band size : 121 kDa
      Additional bands at : 50 kDa (possible cleavage fragment,cross reactivity,truncated form,non-specific binding,degradation product).

    References for Anti-REST / NRSF antibody (ab28018)

    This product has been referenced in:
    • Lu T  et al. REST and stress resistance in ageing and Alzheimer's disease. Nature 507:448-54 (2014). Read more (PubMed: 24670762) »
    • Liang H  et al. CtBP2 Downregulation during Neural Crest Specification Induces Expression of Mitf and REST, Resulting in Melanocyte Differentiation and Sympathoadrenal Lineage Suppression. Mol Cell Biol 31:955-70 (2011). WB ; Quail . Read more (PubMed: 21199918) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (7.5%)
    Sample Pig Tissue lysate - whole (Heart)
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 08 2014

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Jun 08 2012

    Je viens de recevoir les derniers résultats en Western Blotpour l'anticorps ab28018.

    L'image est en pièce jointe, les détails de chaque puits sont les suivants :

    Conditions expérimentales:

    Lanes...

    Read More

    Merci de nous avoir contactés.

    Ma collègue du laboratoire m'a informé que de récents tests ont montré une bonne activité de cet anticorps, même lot que celui que vous avez reçu et utilis&...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Mouse Tissue sections (brain sections)
    Specification brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    Submitted Oct 13 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"