Anti-REST / NRSF antibody [EPR2436Y] (ab75785)


  • Product nameAnti-REST / NRSF antibody [EPR2436Y]
    See all REST / NRSF primary antibodies
  • Description
    Rabbit monoclonal [EPR2436Y] to REST / NRSF
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human REST/ NRSF

  • Positive control
    • Raji and HeLa cell lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab75785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 132 kDa.Can be blocked with REST / NRSF peptide (ab202336).
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionTranscriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
    • Tissue specificityUbiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
    • Sequence similaritiesContains 9 C2H2-type zinc fingers.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Neural Restrictive Silencer Factor antibody
      • Neural-restrictive silencer factor antibody
      • Neuron Restrictive Silencer Factor antibody
      • NRSF antibody
      • RE1 silencing transcription factor antibody
      • RE1-silencing transcription factor antibody
      • Repressor binding to the X2 box antibody
      • rest antibody
      • REST protein antibody
      • REST_HUMAN antibody
      • X2 box repressor antibody
      • XBR antibody
      see all

    Anti-REST / NRSF antibody [EPR2436Y] images

    • All lanes : Anti-REST / NRSF antibody [EPR2436Y] (ab75785) at 1/2000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 132 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)

    References for Anti-REST / NRSF antibody [EPR2436Y] (ab75785)

    This product has been referenced in:
    • Ma P  et al. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A 109:E481-9 (2012). WB ; Mouse . Read more (PubMed: 22223663) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 90 µg
    Gel Running Conditions Reduced Denaturing (4-18% gel)
    Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification mouse embryonic stem cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 17 2015

    Thank you for contacting us.

    Any general Flow Cytometry protocol would work for this antibody. Please check the protocol below for guideline;

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