Recombinant
RabMAb

Anti-REST / NRSF antibody [EPR2436Y] (ab75785)

Overview

  • Product name
    Anti-REST / NRSF antibody [EPR2436Y]
    See all REST / NRSF primary antibodies
  • Description
    Rabbit monoclonal [EPR2436Y] to REST / NRSF
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human REST/ NRSF aa 100-200 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab202336)

  • Positive control
    • Raji and HeLa cell lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 132 kDa.Can be blocked with REST / NRSF peptide (ab202336).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
    • Tissue specificity
      Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.
    • Sequence similarities
      Contains 9 C2H2-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Neural Restrictive Silencer Factor antibody
      • Neural-restrictive silencer factor antibody
      • Neuron Restrictive Silencer Factor antibody
      • NRSF antibody
      • RE1 silencing transcription factor antibody
      • RE1-silencing transcription factor antibody
      • Repressor binding to the X2 box antibody
      • rest antibody
      • REST protein antibody
      • REST_HUMAN antibody
      • X2 box repressor antibody
      • XBR antibody
      see all

    Images

    • All lanes : Anti-REST / NRSF antibody [EPR2436Y] (ab75785) at 1/2000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 132 kDa
      Observed band size: 160 kDa (why is the actual band size different from the predicted?)

    • Anti-REST / NRSF antibody [EPR2436Y] (ab75785) at 1/2000 dilution + His-Tagged human REST (aa 101 to 300) recombinant protein, 10ng

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 132 kDa
      Observed band size: 27 kDa (why is the actual band size different from the predicted?)


      Exposure time: 8 seconds


      Blocking/Diluting buffer and concentration 5% NFDM/TBST

    References

    This product has been referenced in:
    • Bire S  et al. Mariner Transposons Contain a Silencer: Possible Role of the Polycomb Repressive Complex 2. PLoS Genet 12:e1005902 (2016). Read more (PubMed: 26939020) »
    • Ma P  et al. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A 109:E481-9 (2012). WB ; Mouse . Read more (PubMed: 22223663) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    90 µg
    Gel Running Conditions
    Reduced Denaturing (4-18% gel)
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells)
    Specification
    mouse embryonic stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Mar 17 2015

    Thank you for contacting us.

    Any general Flow Cytometry protocol would work for this antibody. Please check the protocol below for guideline;

    http://www.epitomics.com/products/support/fc

    I hope this information is helpful to ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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