Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)

Overview

  • Product name
    Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7]
  • Description
    Rat monoclonal [ER-TR7] to Reticular Fibroblasts and Reticular Fibres
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Murine thymic reticulum

  • Epitope
    The antigen has not been not fully characterized. The recognized epitope may be part of reticulin.
  • Positive control
    • Mouse spleen, reticular fibroblasts and reticular fibres, Human tonsil.
  • General notes
    Antigen Distribution: Isolated cells: The antigen is found in the cytoplasm of fibroblasts and fibroblast cell lines. Tissue sections: In all organs tested so far, the antigen is expressed in connective tissues which form a supporting network between parenchymal cells (see table below). Thus, the supportive mesenchymal structures of larger vessels can be studied. In spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicules, paracortex and medullary cords are also clearly delineated in lymph nodes.


    This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, 5mg/ml BSA, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.
  • Clonality
    Monoclonal
  • Clone number
    ER-TR7
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use a concentration of 1 - 4 µg/ml.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Relevance
      Connective tissue consists of a ground of glycosaminoglycans, proteoglycans and glycoproteins through which various fibres run. These fibres can be collagenous, elastic or reticular. Reticular fibres are composed from the family of collagen proteins and give tensile strength. These fibres are made by reticular fibroblasts.

    Images

    • Immunohistochemical analysis of Mouse liver frozen section, labeling reticular fibres with ab51824.

    • ab51824 staining Reticular Fibroblasts and Reticular Fibres in Mouse spleen tissue sections by Immunohistochemistry (Frozen sections). The sections were fixed with 4% PFA for 10 minutes at room temperature prior to blocking with 10% Goat serum for 30 minutes at 15°C. The primary antibody was diluted 1/500 in PBS with 2% Goat serum and incubated with the sample for 1 hour at 15°C. An Alexa Fluor® 488-conjugated Goat anti-Rat IgG polyclonal was used as the secondary antibody, diluted 1/500.

      See Abreview

    • ab51824 at 1 µg/ml staining mouse spleen; frozen section.
    • Overlay histogram showing HT1080 cells stained with ab51824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51824 , 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758](ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    • Immunohistochemical analysis of Human skin frozen section, labeling reticular fibres with ab51824.

    References

    This product has been referenced in:
    • Seedhom MO  et al. Protein Translation Activity: A New Measure of Host Immune Cell Activation. J Immunol 197:1498-506 (2016). Read more (PubMed: 27385780) »
    • Goldmann T  et al. Origin, fate and dynamics of macrophages at central nervous system interfaces. Nat Immunol 17:797-805 (2016). IHC-P ; Human . Read more (PubMed: 27135602) »

    See all 23 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 16 2016

    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Meninges-Dura Mater)
    Specification
    Meninges-Dura Mater
    Username

    Abcam user community

    Verified customer

    Submitted Apr 18 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Sample
    Pig Cultured Cells (kidney)
    Specification
    kidney
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Dr. Zheng Yu Wang

    Verified customer

    Submitted Sep 24 2013



    This antibody reacts with porcine tissue. However, the sequence is not known.

    Thank you for your enquiry and your interest in our products.

    The tested applications and the species are normally listed on the on-line product datasheets. Generally, we test our antibodies in human, mouse and rat samples - very few antibodi...

    Read More

    Thank you for your enquiry.

    I can confirm that ab51824 has been tested on frozen section sections (IHC-Fr), so would be covered by our guarantee for frozen sections in IF.

    However, as far as we are aware, ithas never been tested i...

    Read More

    Thank you for contacting us. I would recommend the Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] ab51824 (www.abcam.com/ab51824) since this product does not react with keratinocytes. As shown on the datasheet it has been tested in ...

    Read More

    L'anticorps monoclonal ab51824, clone [ER-TR7], reconnait un antigène présent et produit par les fibroblasts réticulaires. Cet anticorps permet le marquage spécifique du tissu conjonctif des organes lymphoïdes.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 15°C
    Username

    Dr. Lynda Coughlan

    Verified customer

    Submitted Dec 15 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Cell (skin)
    Specification
    skin
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 20 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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