Anti-Reticulon 1A antibody [MON160] (ab8957)
Key features and details
- Mouse monoclonal [MON160] to Reticulon 1A
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Reticulon 1A antibody [MON160]
See all Reticulon 1A primary antibodies -
Description
Mouse monoclonal [MON160] to Reticulon 1A -
Host species
Mouse -
Specificity
This antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Reticulon 1A aa 1-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).
Database link: Q16799 -
Epitope
This antibody is reactive with residues 174-337 of Reticulon-1A. -
General notes
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution. -
Clonality
Monoclonal -
Clone number
MON160 -
Myeloma
unknown -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. |
Notes |
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IHC-P
Use at an assay dependent concentration. Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. |
Target
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Relevance
Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing. -
Cellular localization
Endoplasmic reticulum -
Database links
- Entrez Gene: 6252 Human
- Omim: 600865 Human
- SwissProt: Q16799 Human
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Alternative names
- Neuroendocrine specific protein antibody
- NSP antibody
- Reticulon1 antibody
- RTN1 antibody
Images
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NSP-A immunostaining in human cerebellum using ab8957.
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Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.
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Schematic representation of of the deduced domain structure of the three NSP-gene encoded proteins, NSP-A, NSP-B and NSP-C and mapping of the epitopes recognized by the different NSP monoclonal antibodies.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab8957 has been referenced in 3 publications.
- Fan Y et al. Inhibition of Reticulon-1A-Mediated Endoplasmic Reticulum Stress in Early AKI Attenuates Renal Fibrosis Development. J Am Soc Nephrol 28:2007-2021 (2017). PubMed: 28137829
- Xiao W et al. Knockdown of RTN1A attenuates ER stress and kidney injury in albumin overload-induced nephropathy. Am J Physiol Renal Physiol 310:F409-15 (2016). PubMed: 26739891
- Fan Y et al. RTN1 mediates progression of kidney disease by inducing ER stress. Nat Commun 6:7841 (2015). PubMed: 26227493