Overview

  • Product name
    Anti-Reticulon 1A antibody [MON160]
    See all Reticulon 1A primary antibodies
  • Description
    Mouse monoclonal [MON160] to Reticulon 1A
  • Specificity
    This antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
  • Immunogen

    Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

  • Epitope
    This antibody is reactive with residues 174-337 of Reticulon-1A.
  • General notes


    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
  • Clonality
    Monoclonal
  • Clone number
    MON160
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/50 - 1/500.

Target

  • Relevance
    Reticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.
  • Cellular localization
    Endoplasmic reticulum
  • Database links
  • Alternative names
    • Neuroendocrine specific protein antibody
    • NSP antibody
    • Reticulon1 antibody
    • RTN1 antibody

Images

  • NSP-A immunostaining in human cerebellum using ab8957.

  • Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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