Anti-Reticulon 1A antibody [MON160] (ab8957)

Overview

  • Product nameAnti-Reticulon 1A antibody [MON160]
    See all Reticulon 1A primary antibodies
  • Description
    Mouse monoclonal [MON160] to Reticulon 1A
  • SpecificityThis antibody exclusively recognizes the 135 kDa Reticulon-1A protein in immunoblots of NCI-H82 and other SCLC cell lines, and stains normal and pathological neural and neuroendocrine tissues
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, ICC, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human
  • Immunogen

    Partially purified bacterially expressed Reticulon-1A (NSP-A) hybrid protein (beta-GAL-NSP-A 6-776).

  • EpitopeThis antibody is reactive with residues 174-337 of Reticulon-1A.
  • General notes


    A central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesA central structure in the secretory pathway of the cell comprises an elaborate endomembranous system with the multifunctional endoplasmic reticulum (ER) as a major constituent. Newly synthesized secretory proteins and a variety of resident proteins of the pathway enter the rough endoplasmic reticulum. Subsequently, the proteins may be sorted selectively to different membrane-delimited organelles including the Golgi apparatus, endosomes, lysosomes and secretory vesicles. Abcam provides antibodies to a new family of ER-associated proteins, the reticulons, which show a tissue specific distribution.
  • ClonalityMonoclonal
  • Clone numberMON160
  • Myelomaunknown
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Recommended range is 1/50 - 1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/50 - 1/500.

Target

  • RelevanceReticulon 1A may be involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. It is expressed in neural and neuroendocrine tissues and cell cultures derived therefrom. Expression of isoform RTN1-C is strongly correlated with neuronal differentiation. Different isoforms have been described as a result of alternative splicing.
  • Cellular localizationEndoplasmic reticulum
  • Database links
  • Alternative names
    • Neuroendocrine specific protein antibody
    • NSP antibody
    • Reticulon1 antibody
    • RTN1 antibody

Anti-Reticulon 1A antibody [MON160] images

  • NSP-A immunostaining in human cerebellum using ab8957.

  • Human carcinoid (A) and small cell lung carcinoma (B) in paraffin sections, immunostained for NSP-A with ab8957.

References for Anti-Reticulon 1A antibody [MON160] (ab8957)

ab8957 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab8957.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"