The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use a concentration of 3 µg/ml.
Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
FunctionE3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth.
Tissue specificityHighly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.
PathwayProtein modification; protein ubiquitination.
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Retinoblastoma-binding protein 6 antibody
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Anti-Retinoblastoma binding protein 6 antibody images
Flow Cytometry - Anti-Retinoblastoma binding protein 6 antibody (ab55787)
Overlay histogram showing Y79 cells stained with ab55787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55787, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
IHC-P - Retinoblastoma binding protein 6 antibody (ab55787)
Retinoblastoma binding protein 6 antibody (ab55787) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
Western blot - Retinoblastoma binding protein 6 antibody (ab55787)
Western blot against tagged recombinant protein immunogen using ab55787 Retinoblastoma binding protein 6 antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
References for Anti-Retinoblastoma binding protein 6 antibody (ab55787)
has not yet been referenced specifically in any publications.
Publishing research using ab55787? Please let us know so that we can cite the reference in this datasheet.
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