Anti-retinoblastoma like 2 antibody - C-terminal (ab189647)
Key features and details
- Rabbit polyclonal to retinoblastoma like 2 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-retinoblastoma like 2 antibody - C-terminal -
Description
Rabbit polyclonal to retinoblastoma like 2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human retinoblastoma like 2 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). (NP_005602.3)
Database link: Q08999 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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Purification notes
ab189647 has been delipidated and defibrinated. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
Retinoblastoma like 2 is directly involved in heterochromatin formation and probably acts as a transcription repressor by recruiting chromatin modifying enzymes to promoters. It is a potent inhibitor of E2F mediated transactivation and associates preferentially with E2F5. It also binds to cyclins A and E. It binds to and may be involved in the transforming capacity of the adenovirus E1A protein. It may also act as a tumor suppressor. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 5934 Human
- Omim: 180203 Human
- SwissProt: Q08999 Human
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Alternative names
- 130 kDa retinoblastoma associated protein antibody
- FLJ26459 antibody
- H sapiens p130 mRNA for 130K protein antibody
see all
Images
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human brain cerebellum sections labeling retinoblastoma like 2 using ab189647 at a 1/500 dilution. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab189647 has been referenced in 1 publication.
- Li Y et al. Exploring Somatic Alteration Associating With Aggressive Behaviors of Papillary Thyroid Carcinomas by Targeted Sequencing. Front Oncol 11:722814 (2021). PubMed: 34692499