Anti-retinoblastoma like 2 antibody - C-terminal (ab189647)

Overview

  • Product name
    Anti-retinoblastoma like 2 antibody - C-terminal
  • Description
    Rabbit polyclonal to retinoblastoma like 2 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human retinoblastoma like 2 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). (NP_005602.3)
    Database link: Q08999

  • Positive control
    • Human brain cerebellum tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purification notes
    ab189647 has been delipidated and defibrinated.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/100.
WB 1/500 - 1/2000. Predicted molecular weight: 128 kDa.

Target

  • Relevance
    Retinoblastoma like 2 is directly involved in heterochromatin formation and probably acts as a transcription repressor by recruiting chromatin modifying enzymes to promoters. It is a potent inhibitor of E2F mediated transactivation and associates preferentially with E2F5. It also binds to cyclins A and E. It binds to and may be involved in the transforming capacity of the adenovirus E1A protein. It may also act as a tumor suppressor.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • 130 kDa retinoblastoma associated protein antibody
    • FLJ26459 antibody
    • H sapiens p130 mRNA for 130K protein antibody
    • P130 antibody
    • PRB2 antibody
    • RB2 antibody
    • RBL 2 antibody
    • RBL2 antibody
    • RBR 2 antibody
    • RBR2 antibody
    • Retinoblastoma like 2 (p130) antibody
    • Retinoblastoma like 2 antibody
    • Retinoblastoma like protein 2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded Human brain cerebellum sections labeling retinoblastoma like 2 using ab189647 at a 1/500 dilution. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

References

ab189647 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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