Anti-Retinoic Acid Receptor beta antibody (ab15515)

Overview

  • Product name
    Anti-Retinoic Acid Receptor beta antibody
    See all Retinoic Acid Receptor beta primary antibodies
  • Description
    Rabbit polyclonal to Retinoic Acid Receptor beta
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)

  • Positive control
    • Testis and heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab15515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • Involvement in disease
    Microphthalmia, syndromic, 12
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Cytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HAP antibody
    • HBV-activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB2 antibody
    see all

Images

  • ab15515 staining Reinoic Acid Receptor beta at 1/100 dilution in human testis by Immunohistochemistry (FFPE-sections).
  • ab15515 at 1/100 staining hamster CHO-K1 cells expressing human retinoic acid receptor by ICC/IF. The cells were formaldehyde fixed and blocked before incubation with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Anti-Retinoic Acid Receptor beta antibody (ab15515) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References

This product has been referenced in:
  • Childs AJ  et al. Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. PLoS One 6:e20249 (2011). IHC-P ; Human . Read more (PubMed: 21674038) »
  • Fabricius EM  et al. Model examination of chemoprevention with retinoids in squamous cell carcinomas of the head and neck region and suitable biomarkers for chemoprevention. Int J Oncol 39:1083-97 (2011). Read more (PubMed: 21785820) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO-K1 cells expressing Human Retinoic Acid Recept)
Specification
CHO-K1 cells expressing Human Retinoic Acid Recept
Fixative
Formaldehyde
Blocking step
TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. Mahesh Mathrubutham

Verified customer

Submitted Aug 21 2007

To our knowledge, this antibody has not yet been tested in WB.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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