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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (N terminal)
Our Abpromise guarantee covers the use of ab15515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).|
ab15515 at 1/100 staining hamster CHO-K1 cells expressing human retinoic acid receptor by ICC/IF. The cells were formaldehyde fixed and blocked before incubation with the antibody for 1 hour at 22°C. A FITC labelled goat anti-rabbit antibody was used as the secondary.