Anti-Retinoic Acid Receptor gamma antibody (ab97569)

Overview

  • Product name
    Anti-Retinoic Acid Receptor gamma antibody
    See all Retinoic Acid Receptor gamma primary antibodies
  • Description
    Rabbit polyclonal to Retinoic Acid Receptor gamma
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Zebrafish
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 119 - 439 of Human Retinoic Acid Receptor gamma (NP_000957).

  • Positive control
    • WB: 293T, A431, H1299, HeLaS3, Raji and NIH3T3 cell lysates IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR1B3 antibody
    • Nuclear receptor subfamily 1 group B member 3 antibody
    • RAR gamma antibody
    • RAR-gamma antibody
    • RARC antibody
    • Rarg antibody
    • RARG_HUMAN antibody
    • Retinoic acid receptor gamma 2 antibody
    • Retinoic acid receptor gamma antibody
    • Retinoic acid receptor, gamma antibody
    see all

Images

  • Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • Anti-Retinoic Acid Receptor gamma antibody (ab97569) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • ab97569, at a 1/200 dilution, staining Retinoic Acid Receptor gamma in paraformaldehyde-fixed HeLa cells, by Immunofluorescence analysis. Right image is merged with a DNA probe.

References

ab97569 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (F9 teratocarcinoma stem cells)
Specification
F9 teratocarcinoma stem cells
Treatment
vehicle control (EtOH) and all-trans retinoic acid (atRA, 1uM, 24hrs)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Dan Stummer

Verified customer

Submitted Mar 28 2014

Thank you for your phone call.
DISCOUNT CODE: xxx
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I am very pleased to hear you would like to accept our offer and test ab97569 in ChIP. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To...

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Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results.

From the WB image sent, it does look like you're getting a strong specific band at 50 kDa, which is the predicted MW for RA...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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