Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
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Overview
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Product nameAnti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal
See all Retinoic Acid Receptor gamma primary antibodies -
DescriptionRabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Retinoic Acid Receptor gamma aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P13631-2 -
Positive control
- NCCIT, NTERA2/D1 and HeLa cell lysates; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR2020(N)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab187159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). | |
| ICC/IF | 1/100 - 1/250. |
Target
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FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.
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Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5916 Human
- Omim: 180190 Human
- SwissProt: P13631 Human
- Unigene: 1497 Human
- Unigene: 733399 Human
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Alternative names
- NR1B3 antibody
- Nuclear receptor subfamily 1 group B member 3 antibody
- RAR gamma antibody
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Images
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![Western blot - Anti-Retinoic Acid Receptor gamma [EPR2020(N)] antibody - N-terminal (ab187159)](http://a.abcam.com/ps/products/187/ab187159/Images/ab187159-220344-ab187159.jpg)
Western blot - Anti-Retinoic Acid Receptor gamma [EPR2020(N)] antibody - N-terminal (ab187159)All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution
Lane 1 : NCCIT cell lysate
Lane 2 : NTERA2/D1 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma [EPR2020(N)] antibody - N-terminal (ab187159)](http://a.abcam.com/ps/products/187/ab187159/Images/ab187159-220346-ttttt.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma [EPR2020(N)] antibody - N-terminal (ab187159)Immunofluorescent analysis of HeLa cells labeling Retinoic Acid Receptor gamma with ab187159 at 1/250 and DAPI staining (blue).
References
ab187159 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"