Anti-Retinoid X Receptor gamma antibody (ab15518)

Overview

  • Product nameAnti-Retinoid X Receptor gamma antibody
    See all Retinoid X Receptor gamma primary antibodies
  • Description
    Rabbit polyclonal to Retinoid X Receptor gamma
  • Tested applicationsSuitable for: IHC-Fr, IHC - Wholemount, ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Human
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Mouse) (N terminal).

  • Positive control
    • Breast carcinoma and prostate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15518 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
IHC - Wholemount Use at an assay dependent concentration.
ICC/IF 1/100.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 51 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC109416 antibody
    • NR2B3 antibody
    • Nuclear receptor subfamily 2 group B member 3 antibody
    • OTTHUMP00000060418 antibody
    • Retanoic X receptor gamma antibody
    • Retinoic acid receptor RXR gamma antibody
    • Retinoic acid receptor RXR-gamma antibody
    • Retinoid X receptor gamma antibody
    • RXR G antibody
    • RXR gamma antibody
    • RXRC antibody
    • Rxrg antibody
    • RXRG_HUMAN antibody
    • RXRgamma antibody
    see all

Anti-Retinoid X Receptor gamma antibody images

  • IHC - Wholemount of Chicken inner ear tissue labelling Retinoid X Receptor gamma with ab15518. Sample was incubated with primary antibody (1/500 in 0.2% Triton-X in PBS) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab15518 staining Retinoid X Receptor gamma in breast carcinoma by Immunohistochemistry (FFPE-sections).
  • Immunostaining of Retinoid X receptor gamma in adult mouse retina using ab15518. Visible are the outer nuclear layer (top) and inner nuclear layer (bottom) of a adult mouse retina. Retionoid X receptor gamma staining was observed in the cone photoreceptors in the outer nuclear layer and also in most if not all cells of the inner nuclear layer. Please see abreview for protocol details.

    See Abreview

  • Anti-Retinoid X Receptor gamma antibody (ab15518) at 1/25 dilution + NIH3T3 cell lysate

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-Retinoid X Receptor gamma antibody (ab15518)

This product has been referenced in:
  • Nickkho-Amiry M  et al. Peroxisome proliferator-activated receptors modulate proliferation and angiogenesis in human endometrial carcinoma. Mol Cancer Res 10:441-53 (2012). WB, IHC-P ; Human . Read more (PubMed: 22205725) »
  • Goonetilleke UR  et al. Death is associated with complement C3 depletion in cerebrospinal fluid of patients with pneumococcal meningitis. MBio 3: (2012). WB ; Human . Read more (PubMed: 22415003) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing
Sample Rat Cell lysate - whole cell (Schwann cells)
Specification Schwann cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Feb 27 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Mouse, whole brain sections)
Specification Mouse, whole brain sections
Permeabilization Yes - Tween-20
Fixative Paraformaldehyde
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Submitted Jan 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Chicken Tissue (Inner Ear Tissue)
Specification Inner Ear Tissue
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Submitted Jun 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (adult retina)
Specification adult retina
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton-x100
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
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Submitted Feb 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (RXRg expression in U2OS)
Specification RXRg expression in U2OS
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
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Dr. Mahesh Mathrubutham

Verified customer

Submitted Feb 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Hamster Cell (CHO K1 cells expressing Human Retinoid X Receptor)
Specification CHO K1 cells expressing Human Retinoid X Receptor
Fixative Formaldehyde
Permeabilization Yes - 0.1 % Triton X-100
Blocking step TBS + 3% Dried Milk + 0.1 % Triton X-100 as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 22°C
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Dr. Mahesh Mathrubutham

Verified customer

Submitted Sep 05 2007

I'm very sorry to hear you are experiencing problems with our RXR and RAR antibodies, thank you for sending us an image of your blots, I understand your problem. The difference in MW can be slightly different between predicted and detected MW and w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"