Overview

  • Product name
    Anti-Retinoid X Receptor gamma antibody
    See all Retinoid X Receptor gamma primary antibodies
  • Description
    Rabbit polyclonal to Retinoid X Receptor gamma
  • Specificity
    ab53162 detects endogenous levels of total Retinoic X Receptor gamma protein.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human Retinoic X Receptor gamma.

  • Positive control
    • HepG2 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab53162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ELISA 1/10000.
IHC-P Use a concentration of 2 - 4 µg/ml.

Target

  • Function
    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC109416 antibody
    • NR2B3 antibody
    • Nuclear receptor subfamily 2 group B member 3 antibody
    • OTTHUMP00000060418 antibody
    • Retanoic X receptor gamma antibody
    • Retinoic acid receptor RXR gamma antibody
    • Retinoic acid receptor RXR-gamma antibody
    • Retinoid X receptor gamma antibody
    • RXR G antibody
    • RXR gamma antibody
    • RXRC antibody
    • Rxrg antibody
    • RXRG_HUMAN antibody
    • RXRgamma antibody
    see all

Images

  • Ab53162 staining Retinoid X Receptor gamma in huma nucleus accumbens.
    Left panel: with primary antibody at a dilution of 4µg/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH9.0 in a Dako PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:
  • Donmez G  et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . Read more (PubMed: 20655472) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
IHC - Wholemount
Sample
Locusta migratoria Embryo (Nervous system)
Specification
Nervous system
Username

Abcam user community

Verified customer

Submitted Apr 10 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293T, HepG2, MEF)
Loading amount
20 µg
Specification
293T, HepG2, MEF
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

Submitted Jan 21 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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