Anti-Retinoid X Receptor gamma antibody (ab53162)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-Retinoid X Receptor gamma antibody
See all Retinoid X Receptor gamma primary antibodies -
DescriptionRabbit polyclonal to Retinoid X Receptor gamma
-
Host speciesRabbit
-
Specificityab53162 detects endogenous levels of total Retinoic X Receptor gamma protein.
-
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
-
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide derived from human Retinoic X Receptor gamma.
-
Positive control
- HepG2 cell extracts.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesab53162 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab53162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). | |
| ELISA | 1/10000. | |
| IHC-P | Use a concentration of 2 - 4 µg/ml. |
Target
-
FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
-
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
-
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 6258 Human
- Entrez Gene: 20183 Mouse
- Entrez Gene: 83574 Rat
- Omim: 180247 Human
- SwissProt: P48443 Human
- SwissProt: P28705 Mouse
- SwissProt: Q5BJR8 Rat
- Unigene: 26550 Human
see all -
Alternative names
- MGC109416 antibody
- NR2B3 antibody
- Nuclear receptor subfamily 2 group B member 3 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Retinoid X Receptor gamma antibody (ab53162)Ab53162 staining Retinoid X Receptor gamma in huma nucleus accumbens.
Left panel: with primary antibody at a dilution of 4µg/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH9.0 in a Dako PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References
This product has been referenced in:
- Donmez G et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . Read more (PubMed: 20655472) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"