Anti-Retinoid X Receptor gamma antibody, prediluted (ab15519)

Overview

  • Product nameAnti-Retinoid X Receptor gamma antibody, prediluted
    See all Retinoid X Receptor gamma primary antibodies
  • Description
    Rabbit polyclonal to Retinoid X Receptor gamma, prediluted
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)(Mouse)(N terminal)

  • Positive control
    • Breast carcinoma and prostate.

Properties

Applications

Our Abpromise guarantee covers the use of ab15519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Ready to use. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (Citrate buffer 10mM pH6.0 for pretreatment--boil for 10min, slowly; cool down for at least 20min). Incubate antibody for 10-30min at RT, use biotin-streptavidin enzymatic detection.
WB Use at an assay dependent concentration. Predicted molecular weight: 51 kDa.

Target

  • FunctionReceptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC109416 antibody
    • NR2B3 antibody
    • Nuclear receptor subfamily 2 group B member 3 antibody
    • OTTHUMP00000060418 antibody
    • Retanoic X receptor gamma antibody
    • Retinoic acid receptor RXR gamma antibody
    • Retinoic acid receptor RXR-gamma antibody
    • Retinoid X receptor gamma antibody
    • RXR G antibody
    • RXR gamma antibody
    • RXRC antibody
    • Rxrg antibody
    • RXRG_HUMAN antibody
    • RXRgamma antibody
    see all

Anti-Retinoid X Receptor gamma antibody, prediluted images

  • ab15519 staining Retinoid X Receptor gamma in Human prostate carcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab15519 staining Retinoid X Receptor gamma in breast carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-Retinoid X Receptor gamma antibody (ab15518) at 1/25 dilution + NIH3T3 cell lysate

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References for Anti-Retinoid X Receptor gamma antibody, prediluted (ab15519)

ab15519 has not yet been referenced specifically in any publications.

Product Wall

We unfortunately do not know of published references with ab15519. The laboratory has used te following protocol sucessfuly: 1. Use breast cancer tissue as positive control 2. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue sec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"