Retinol binding protein 4 (RBP4) Human ELISA Kit (ab108897)
- Product nameRetinol binding protein 4 (RBP4) Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.7%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
- Assay typeSandwich (quantitative)
- Sensitivity= 6 ng/ml
- Range6.25 ng/ml - 400 ng/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Retinol binding protein 4 (RBP4) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein 4 concentrations in plasma, serum, saliva, urine milk and cell culture supernatants.
A Retinol binding protein 4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein 4specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein 4 captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Retinol binding Protein 4 Antibody 1 x 140µl Chromogen Substrate 1 x 8ml Retinol binding Protein 4 Microplate (12 x 8 well strips) 1 unit Retinol binding Protein 4 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
- Research Areas
- SwissProt: P02753 Human
Our Abpromise guarantee covers the use of ab108897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Retinol binding protein 4 (RBP4) Human ELISA Kit images
Representative Standard Curve Using ab108897.
References for Retinol binding protein 4 (RBP4) Human ELISA Kit (ab108897)
ab108897 has not yet been referenced specifically in any publications.