Retinol binding protein (RBP) Human ELISA Kit (ab108899)
- Product nameRetinol binding protein (RBP) Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 4.6%
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Milk, Urine
- Assay typeSandwich (quantitative)
- Sensitivity0.008 µg/ml
- Range0.008 µg/ml - 0.5 µg/ml
- Assay time4h 00m
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s Retinol binding protein (RBP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein concentrations in Human milk, urine and cell culture supernatants.
A Retinol binding protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein captured in plate.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate (SP Conjugate) 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human RBP Antibody 1 x 130µl Chromogen Substrate 1 x 8ml Retinol binding protein Microplate (12 x 8 well strips) 1 unit Retinol binding protein Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
Our Abpromise guarantee covers the use of ab108899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Retinol binding protein (RBP) Human ELISA Kit (ab108899)
ab108899 has not yet been referenced specifically in any publications.