Retinol binding protein (RBP) Human ELISA Kit (ab108899)

Overview

  • Product nameRetinol binding protein (RBP) Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.6%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine
  • Assay typeSandwich (quantitative)
  • Sensitivity
    0.008 µg/ml
  • Range
    0.008 µg/ml - 0.5 µg/ml
  • Recovery

    98 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Retinol binding protein (RBP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein concentrations in Human milk, urine, CSF, saliva and cell culture supernatants.

    A Retinol binding protein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent dilution.

Retinol binding protein (RBP) Human ELISA Kit images

  • Representative Standard Curve using ab108899.

Protocols

References for Retinol binding protein (RBP) Human ELISA Kit (ab108899)

ab108899 has not yet been referenced specifically in any publications.

Product Wall

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The reference range for retinol binding protein in urine sample using the Retinol binding protein Human ELISA Kit (ab108899) has not been determined. The kit has been used with uri...

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Thank you for contacting us and sorry for the delay in getting back to you in regards to the Retinol binding protein Human ELISA Kit (ab108899).

I now have confirmation from the source of this kit that with urine samples, you should not need ...

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I am in contact with our marketing team and will get back to you with a quote as soon as possible. I will also let you know as soon as I have confirmation of what the best way of preserving the urine samples is.
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Thank you for contacting us yesterday. As discussed over the phone, I am currently seeking further information as to the best method of preparing your urine samples to be used with the Retinol binding protein Human ELISA Kit (ab108899). I will get back...

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The total cost for 1 kits (ab108899) is GBP363 including the packaging and delivery charges.

1 kit will be suitable for 96 reactions in 96 well plate.

Unopened kit is guaranteed for 6 months if s...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"