Anti-Retinol dehydrogenase 16 antibody (ab84344)

Overview

  • Product nameAnti-Retinol dehydrogenase 16 antibody
    See all Retinol dehydrogenase 16 primary antibodies
  • Description
    Rabbit polyclonal to Retinol dehydrogenase 16
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (WLYLAVFVGL YYLLHWYRER QVLSHLRDKY VFITGCDSGF GKLLARQLDA) of Human Retinol dehydrogenase 16 (NP_003699).

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionOxidoreductase with a preference for NAD. Oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. Has higher activity towards CRBP-bound retinol than with free retinol. Oxidizes 3-alpha-hydroxysteroids. Oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. Can also catalyze the reverse reaction.
  • Tissue specificityHighly expressed in adult liver (at protein level). Detected in endometrium, liver and foreskin. Detected in the spineous layers of adult skin, and at lower levels in basal and granular skin layers. Detected in fetal liver and lung.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Post-translational
    modifications
    Not N-glycosylated.
  • Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Microsomal NAD(+)-dependent retinol dehydrogenase 4 antibody
    • Microsomal NAD+ dependent retinol dehydrogenase 4 antibody
    • RDH 16 antibody
    • RDH16 antibody
    • RDH16_HUMAN antibody
    • Retinol dehydrogenase 16 (all trans and 13 cis) antibody
    • Retinol dehydrogenase 16 (all trans) antibody
    • Retinol dehydrogenase 16 antibody
    • RODH 4 antibody
    • RoDH-4 antibody
    • SDR9C8 antibody
    • Short chain dehydrogenase/reductase family 9C member 8 antibody
    • Sterol/retinol dehydrogenase antibody
    see all

Anti-Retinol dehydrogenase 16 antibody images

  • Anti-Retinol dehydrogenase 16 antibody (ab84344) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

References for Anti-Retinol dehydrogenase 16 antibody (ab84344)

ab84344 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Liver, primary hepatocyte)
Gel Running Conditions Reduced Denaturing (10% acrylamide gel)
Loading amount 10 µg
Specification Liver, primary hepatocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 22 2015

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