Anti-REV1 antibody (ab5088)
Key features and details
- Goat polyclonal to REV1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2c
Overview
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Product name
Anti-REV1 antibody
See all REV1 primary antibodies -
Description
Goat polyclonal to REV1 -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog -
Immunogen
Synthetic peptide corresponding to Human REV1 aa 1200 to the C-terminus.
Database link: Q9UBZ9-1 -
Positive control
- IHC-P: Human Testis tissue.
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General notes
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions. -
Clonality
Polyclonal -
Isotype
IgG2c -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the DNA polymerase type-Y family.
Contains 1 BRCT domain.
Contains 1 umuC domain. -
Domain
The C-terminal domain is necessary for protein interactions. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 51455 Human
- Entrez Gene: 56210 Mouse
- Entrez Gene: 316344 Rat
- GenBank: NP_057400 Human
- Omim: 606134 Human
- SwissProt: Q9UBZ9 Human
- SwissProt: Q920Q2 Mouse
- Unigene: 443077 Human
see all -
Alternative names
- AIBP 80 antibody
- AIBP80 antibody
- Alpha integrin binding protein 80 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab5088 has been referenced in 3 publications.
- Goehringer N et al. Improved Anticancer Activities of a New Pentafluorothio-Substituted Vorinostat-Type Histone Deacetylase Inhibitor. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34959719
- Yeo C et al. Epigallocatechin-3-Gallate Suppresses Vasculogenic Mimicry through Inhibiting the Twist/VE-Cadherin/AKT Pathway in Human Prostate Cancer PC-3 Cells. Int J Mol Sci 21:N/A (2020). PubMed: 31936664
- Imani S et al. Prognostic Value of EMT-inducing Transcription Factors (EMT-TFs) in Metastatic Breast Cancer: A Systematic Review and Meta-analysis. Sci Rep 6:28587 (2016). PubMed: 27335258