Anti-REV1 antibody (ab5088)

Overview

  • Product nameAnti-REV1 antibody
    See all REV1 primary antibodies
  • Description
    Goat polyclonal to REV1
  • Tested applicationsSuitable for: ICCmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    VLQQTYGSTLKVT

    , corresponding to amino acids 1239-1251 of Human REV 1L.

  • General notes


    REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notesREV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
  • ClonalityPolyclonal
  • IsotypeIgG2c
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab5088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 4 - 6 µg/ml.

Target

  • FunctionDeoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the DNA polymerase type-Y family.
    Contains 1 BRCT domain.
    Contains 1 umuC domain.
  • DomainThe C-terminal domain is necessary for protein interactions.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AIBP 80 antibody
    • AIBP80 antibody
    • Alpha integrin binding protein 80 antibody
    • Alpha integrin-binding protein 80 antibody
    • DNA repair protein REV 1 antibody
    • DNA repair protein REV1 antibody
    • FLJ21523 antibody
    • MGC163283 antibody
    • MGC26225 antibody
    • REV 1 (yeast homolog) like antibody
    • REV 1 antibody
    • REV 1 homolog antibody
    • REV 1 like antibody
    • REV 1 protein antibody
    • REV 1L antibody
    • REV1 (yeast homolog) like antibody
    • REV1 antibody
    • REV1 homolog (S. cerevisiae) antibody
    • REV1 homolog antibody
    • REV1 like (yeast) antibody
    • REV1 like antibody
    • Rev1 like terminal deoxycytidyl transferase antibody
    • REV1 protein antibody
    • Rev1-like terminal deoxycytidyl transferase antibody
    • REV1_HUMAN antibody
    • REV1L antibody
    see all

Anti-REV1 antibody images

  • In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. ab5088 used at 4ug/ml following Steamed antigen retrieval with Tris/EDTA buffer pH 9 visualised using HRP staining.

References for Anti-REV1 antibody (ab5088)

ab5088 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS)
Permeabilization Yes - NP-40
Specification U2OS
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Formaldehyde
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Verified customer

Submitted May 16 2016

Thank you for your email. Ab5088 is a Fast-Track antibody and has not yet been fully characterized. We are unable to provide a discount, but we will award $300 of Conference Travel Money to the first researcher who sends us positive feedback (including...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"