- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-REV1 antibody
See all REV1 primary antibodies -
DescriptionGoat polyclonal to REV1
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Host speciesGoat
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Tested applicationsSuitable for: ICCmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
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Immunogen
Synthetic peptide:
VLQQTYGSTLKVT
, corresponding to amino acids 1239-1251 of Human REV 1L. -
General notes
REV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Primary antibody notesREV1L is the human homolog of the S. cerevisiae mutagenesis protein Rev1. The REV1 protein is conserved from yeast to humans. All REV1 proteins contain a BRCT domain, which is important in protein-protein interactions.
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ClonalityPolyclonal
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IsotypeIgG2c
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab5088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use a concentration of 4 - 6 µg/ml. |
Target
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FunctionDeoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the DNA polymerase type-Y family.
Contains 1 BRCT domain.
Contains 1 umuC domain. -
DomainThe C-terminal domain is necessary for protein interactions.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 51455 Human
- Entrez Gene: 56210 Mouse
- Entrez Gene: 316344 Rat
- GenBank: NP_057400 Human
- Omim: 606134 Human
- SwissProt: Q9UBZ9 Human
- SwissProt: Q920Q2 Mouse
- Unigene: 443077 Human
see all -
Alternative names
- AIBP 80 antibody
- AIBP80 antibody
- Alpha integrin binding protein 80 antibody
see all
Images
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Immunocytochemistry - Anti-REV1 antibody (ab5088)In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. ab5088 used at 4ug/ml following Steamed antigen retrieval with Tris/EDTA buffer pH 9 visualised using HRP staining.
References
This product has been referenced in:
- Imani S et al. Prognostic Value of EMT-inducing Transcription Factors (EMT-TFs) in Metastatic Breast Cancer: A Systematic Review and Meta-analysis. Sci Rep 6:28587 (2016). Read more (PubMed: 27335258) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"