Anti-REV3L antibody (ab111729)
Key features and details
- Rabbit polyclonal to REV3L
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-REV3L antibody -
Description
Rabbit polyclonal to REV3L -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human REV3L aa 200-300 (internal sequence).
Database link: O60673 -
Positive control
- Human brain tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS (without Mg2+ and Ca2+) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab111729 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab111729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Interacts with MAD2L2 to form the error prone DNA polymerase zeta involved in translesion DNA synthesis. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the DNA polymerase type-B family.
Contains 1 CysA-type zinc finger. -
Domain
Its C-terminal part could serve as the catalytic domain during nucleotide polymerization, while its N-terminal part could provide sites for protein-protein interactions with other factors during translesion DNA synthesis.
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5980 Human
- Entrez Gene: 19714 Mouse
- Omim: 602776 Human
- SwissProt: O60673 Human
- SwissProt: Q61493 Mouse
- Unigene: 232021 Human
- Unigene: 439723 Mouse
-
Alternative names
- Catalytic subunit of DNA polymerase zeta antibody
- DNA polymerase zeta catalytic subunit antibody
- DPOLZ_HUMAN antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab111729 has been referenced in 1 publication.
- Wang D et al. Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma. Open Life Sci 15:488-500 (2020). PubMed: 33817237