Overview

  • Product nameAnti-REV3L antibody
    See all REV3L primary antibodies
  • Description
    Mouse monoclonal to REV3L
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fusion protein: LAGLESDGYR GERNRMPSPC RSFGNNKYPQ NSDDEENEPQ IEKEEMELSL VMSQRWDSNI EEHCAKKRSL CRNTHRSSTE DDDSSSGEEM EWSDNSLLLA , corresponding to amino acids 418-518 of Human REV3L

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityMonoclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 352 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • RelevanceThis Product recognizes the catalytic subunit of DNA polymerase zeta a nonessential enzyme that is thought to carry out translation replication and is responsible for virtually all DNA damage-induced mutagenesis and the majority of spontaneous mutagenesis.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Catalytic subunit of DNA polymerase zeta antibody
    • Polymerase DNA zeta antibody
    • POLZ antibody
    • RAD54 like antibody
    • REV3 yeast homolog like antibody
    • Sez4 antibody
    • Yeast homolog of Rev 3 antibody
    see all

Anti-REV3L antibody images

  • All lanes : Anti-REV3L antibody (ab52704) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100ng of a Tagged irrelevant antigen (Negative control)
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) HRP at 1/5000 dilution

    Predicted band size : 352 kDa

References for Anti-REV3L antibody (ab52704)

ab52704 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"