Overview

  • Product name
  • Description
    Rabbit polyclonal to Rex1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Rex1.

    (Peptide available as ab28619.)

  • Positive control
    • Lysate of 293T cells overexpressing Rex1, Mouse Embryonic Stem Cells expressing biotinylated Rex1.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.
  • Tissue specificity
    Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hREX-1 antibody
    • Reduced Expression 1 Protein antibody
    • Reduced expression protein 1 antibody
    • Rex-1 antibody
    • REX1 antibody
    • REX1 transcription factor antibody
    • Zfp-42 antibody
    • Zfp42 antibody
    • ZFP42 zinc finger protein antibody
    • ZFP42_HUMAN antibody
    • Zinc finger protein 42 homolog antibody
    • Zinc finger protein 754 antibody
    • ZNF754 antibody
    see all

Images

  • ICC/IF image of ab28141 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28141, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • All lanes : Anti-Rex1 antibody (ab28141) at 0.5 µg/ml

    Lane 1 : Lysate of 293T cells overexpressing Rex1
    Lane 2 : Lysate of Mouse Embryonic Stem Cells expressing biotinylated Rex1
    Lane 3 : Negative control for tagged protein - Lysate of Mouse Embryonic Stem Cells (endogenous protein present)
    Lane 4 : Lysate of various passages of Mouse Embryonic Stem Cells expressing biotinylated Rex1
    Lane 5 : Lysate of various passages of Mouse Embryonic Stem Cells expressing biotinylated Rex1
    Lane 6 : Lysate of various passages of Mouse Embryonic Stem Cells expressing biotinylated Rex1
    Lane 7 : Lysate of various passages of Mouse Embryonic Stem Cells expressing biotinylated Rex1


    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 34 kDa (possible non-specific binding),36 kDa (possible non-specific binding),38 kDa (possible tagged protein).
  • Anti-Rex1 antibody (ab28141) at 1 µg/ml + 293T Cells Overexpressing Mouse Rex1 at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Loos F  et al. Xist and Tsix Transcription Dynamics Is Regulated by the X-to-Autosome Ratio and Semistable Transcriptional States. Mol Cell Biol 36:2656-2667 (2016). Read more (PubMed: 27528619) »
  • Chelmicki T  et al. MOF-associated complexes ensure stem cell identity and Xist repression. Elife 3:e02024 (2014). Read more (PubMed: 24842875) »

See all 9 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Sep 15 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton X-100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 02 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Murine Embryonic Stem Cell Lysate)
Loading amount
40 µg
Specification
Murine Embryonic Stem Cell Lysate
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Utkarsh Kapoor

Verified customer

Submitted Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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