Overview

  • Product name
  • Description
    Rabbit polyclonal to Rex1
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab50828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
ICC/IF 1/50.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Involved in self-renewal property of ES cells (By similarity). May be involved in transcriptional regulation.
  • Tissue specificity
    Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.
  • Sequence similarities
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • hREX-1 antibody
    • Reduced Expression 1 Protein antibody
    • Reduced expression protein 1 antibody
    • Rex-1 antibody
    • REX1 antibody
    • REX1 transcription factor antibody
    • Zfp-42 antibody
    • Zfp42 antibody
    • ZFP42 zinc finger protein antibody
    • ZFP42_HUMAN antibody
    • Zinc finger protein 42 homolog antibody
    • Zinc finger protein 754 antibody
    • ZNF754 antibody
    see all

Images

  • Anti-Rex1 antibody (ab50828) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG (1: 50,000 - 100,000)

    Predicted band size : 35 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence on Human Embryonic Stem Cells using Rex1 antibody (ab50828). Left hand image represents REX1 staining detected with AlexaFluor 633 secondary antibody (red). Right hand image represents REX1 staining (red) overlayed by nuclear DAPI staining (blue). See abreview for more details.
  • ab50828 staining Rex1 in human tissue sections (ESC-derived embryoid bodies) by immunohistochemistry (paraformaldehyde fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 8% Serum treatment in automated Bond Max system for 15 minutes. Samples were incubated with primary antibody (1/50) for 15 minutes. An HRP-conjugated goat anti-rabbit polyclonal (1/200) was used as secondary antibody.

    See Abreview

  • Immunofluorescence analysis of Human umbilical cord blood-derived mesenchymal stem cells, staining Rex1 with ab50828.

    Cells were fixed with paraformaldehyde for 20 minutes at room temperature, and incubated with 10% normal goat serum overnight at 4°C. Samples were incubated overnight at 4°C with primary antibody diluted in blocking solution followed by an AlexaFluor® anti-rabbit IgG secondary antibody for 1 hour.

References

This product has been referenced in:
  • Chen H  et al. Inducing goat pluripotent stem cells with four transcription factor mRNAs that activate endogenous promoters. BMC Biotechnol 17:11 (2017). ICC/IF . Read more (PubMed: 28193206) »
  • Mennan C  et al. Mesenchymal stromal cells derived from whole human umbilical cord exhibit similar properties to those derived from Wharton's jelly and bone marrow. FEBS Open Bio 6:1054-1066 (2016). ICC/IF . Read more (PubMed: 27833846) »

See all 8 Publications for this product

Customer reviews and Q&As

Thank you for contacting us and your interest in our products.

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We have not yet tested ab50828 in flow cytometry as yet and therefore do not know how well ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human ES cell)
Loading amount
10 µg
Specification
human ES cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (hESC-derived embryoid bodies)
Specification
hESC-derived embryoid bodies
Fixative
Paraformaldehyde
Antigen retrieval step
Other
Permeabilization
No
Blocking step
Serum provided by automated Bondmax System as blocking agent for 15 minute(s) · Concentration: 8%
Username

Abcam user community

Verified customer

Submitted Apr 21 2010

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonal carcinoma cell lines)
Loading amount
20 µg
Specification
mouse embryonal carcinoma cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Embryonic Stem Cells)
Specification
Human Embryonic Stem Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 for 15min at r.t.
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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